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Literature summary for 3.4.21.25 extracted from

  • Sharma, A.; Kumari, M.; Jagannadham, M.
    Religiosin C, a cucumisin-like serine protease from Ficus religiosa (2012), Process Biochem., 47, 914-921.
No PubMed abstract available
Search for more literature for 3.4.21.25

Application

Application Comment Organism
food industry the high milk-clotting ability of religiosin C supports its use in the food industry Ficus religiosa

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 60.03% residual activity at 50% (v/v) Ficus religiosa
butanol 68.24% residual activity at 50% (v/v) Ficus religiosa
chymostatin 2.45% residual activity at 5 mM Ficus religiosa
diisopropylfluorophosphate 2.34% residual activity at 1 mM Ficus religiosa
dioxane 65.35% residual activity at 50% (v/v) Ficus religiosa
ethanol 75.09% residual activity at 50% (v/v) Ficus religiosa
additional information not inhibited by EDTA, EGTA, iodoacetic acid, 2-phenanthroline, SBTI, and dithiothreitol Ficus religiosa
phenylmethylsulfonyl fluoride 5.12% residual activity at 5 mM Ficus religiosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
0.004
-
 L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
0.093
-
 L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
0.273
-
 L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not influenced by Hg2+, Na+, K+, Mg2+, Ca2+, Rb+, and Li+ Ficus religiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 1 * 80000, SDS-PAGE Ficus religiosa

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme is not influenced by urea, methanol, and guanidine hydrochloride Ficus religiosa

Organism

Organism UniProt Comment Textmining
Ficus religiosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Ficus religiosa

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Ficus religiosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme from crude latex shows a specific activity of 23.99 units/mg at pH 8.0 and 37°C. After 2.29fold purification, the enzyme shows a specific activity of 55.01 units/mg at pH 8.0 and 37°C. One unit of enzyme activity is defined as the amount of enzyme that gives rise to an increase in one unit of absorbance at 280 nm per min of casein digestion Ficus religiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Ficus religiosa ?
-
 ?
L-alanyl-4-nitroanilide + H2O
-
 Ficus religiosa L-alanine + 4-nitroaniline
-
 ?
L-alanyl-L-alanyl-4-nitroanilide + H2O
-
 Ficus religiosa L-alanyl-L-alanine + 4-nitroaniline
-
 ?
L-gamma-glutamyl-4-nitroanilide + H2O
-
 Ficus religiosa L-glutamate + 4-nitroaniline
-
 ?
L-leucyl-4-nitroanilide + H2O
-
 Ficus religiosa L-leucine + 4-nitroaniline
-
 ?
additional information no activity toward Nalpha-benzoyl-DL-arginyl-4-nitroanilide and N-succinyl-L-phenylalanyl-4-nitroanilide Ficus religiosa ?
-
 ?
skim milk + H2O
-
 Ficus religiosa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Ficus religiosa

Synonyms

Synonyms Comment Organism
religiosin C
-
 Ficus religiosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Ficus religiosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 more than 80% of the activity is observed from 40 to 55°C. The activity decreases steadily as the temperature rises over 60°C and no activity is observed at 80°C Ficus religiosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 70 the enzyme shows more than 90% activity up to 60°C for 30 min. 87% of activity is observed at 70°C when incubated for 30 min Ficus religiosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.48
-
 L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
32.13
-
 L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
207.8
-
 L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
749.7
-
 L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Ficus religiosa

pH Range

pH Minimum pH Maximum Comment Organism
3.5 11.5 the enzyme retains more than 80% of activity from pH 3.5 to 11.5. No activity is observed at or below pH 2.0 Ficus religiosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11.5 the enzyme shows more than 90% activity when incubated at pH 4.5 to 11.5 for 30 min Ficus religiosa

pI Value

Organism Comment pI Value Maximum pI Value
Ficus religiosa isoelectric focusing
-
 4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
422.2
-
 L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
749.7
-
 L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
6888
-
 L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
13910
-
 L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa