Literature summary for 3.4.21.25 extracted from

Murayama, K.; Kato-Murayama, M.; Hosaka, T.; Sotokawauchi, A.; Yokoyama, S.; Arima, K.; Shirouzu, M.
Crystal structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L. (2012), J. Mol. Biol., 423, 386-396.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M imidazole buffer (pH 6.5) containing 0.8 M sodium acetate	Cucumis melo

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Cucumis melo	-	-

Organism

Organism	UniProt	Comment	Textmining
Cucumis melo	Q39547	variant Prince	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Cucumis melo

Purification (Commentary)

Purification (Comment)	Organism
HiTrap SP column chromatography and Superdex 200 gel filtration	Cucumis melo

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Leu-Leu-Pro-Met-Ala-Leu + H2O	-	Cucumis melo	Leu-Leu-Pro-Met + Ala-Leu	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
81	-	melting temperature	Cucumis melo

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Release 2023.1 (January 2023)