Application | Comment | Organism |
---|---|---|
medicine | the therapeutic efficacy and safety of recombinant factor VIIa in haemophilia patients with inhibitors is established in a number of clinical trials which also forms the basis for regulatory approval | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
for expression as a recombinant protein in baby hamster kidney cells cDNA comprising the entire FVII open reading frame encoding signal peptide, propeptide and the mature FVII protein is inserted into an expression plasmid containing the elements required to direct transcription of the FVII cDNA in mammalian cells. The endogenous signal peptide and propeptide of FVII are necessary for intracellular transport and processing of FVII in the producer cells but are both removed prior to secretion | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
306X | mutation studies show that the interaction between protein cofactor tissue factor and methionine-306 in the serine protease domain of FVIIa triggers the activation process and suggested some ensuing steps on the pathway to the active conformation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ ions constitute an important cofactor for FVIIa and bind to a total of nine sites, seven in the N-terminal gamma-carboxyglutamic acid (Gla) domain and one each in the first EGF-like and protease domains | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | complex formation with the essential protein cofactor tissue factor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08709 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | FVII is secreted as a single-chain polypeptide and a peptide bond cleavage (after arginine 152) results in the enzymatically active form FVIIa. The two polypeptide chains are referred to as light (152 residues) and heavy (254 residues). The light chain contains an N-terminal gamma-carboxyglutamic acid (Gla) domain, in which all ten Glu residues are posttranslationally-carboxylated | Homo sapiens |
Purification (Comment) | Organism |
---|---|
purification process starts with pH adjustment of the clarified medium, which is loaded onto an anion-exchange column. Detergent is added to the eluate in order to inactivate enveloped viruses. Subsequently, FVII and FVIIa are affinity purified on a column with a monoclonal antibody recognizing the FVII Gla domain in its functional conformation. Only FVII/FVIIa without propetide and with a functional and sufficiently gamma-carboxylated Gla domain is captured and purified. The eluate from the affinity column is subjected to two anion-exchange chromatography steps | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | complex formation with the essential protein cofactor tissue factor | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor VIIa | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | complex formation with the essential protein cofactor tissue factor | Homo sapiens |