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Literature summary for 3.4.21.21 extracted from

  • Petrovan, R.J.; Ruf, W.
    Residue Met(156) contributes to the labile enzyme conformation of coagulation factor VIIa (2001), J. Biol. Chem., 276, 6616-6620.
    View publication on PubMed
Search for more literature for 3.4.21.21

Protein Variants

Protein Variants Comment Organism
M156Q mutation has no influence on the amidolytic and proteolytic activity of tissue factor bound enzyme, increased affinity for tissue factor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme, addition of tissue factor Homo sapiens
1.2
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor Homo sapiens
5.3
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q Homo sapiens
7.6
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.4, 37°C Homo sapiens
61
-
 Factor X pH 7.4, 37°C, wild-type enzyme Homo sapiens
68
-
 Factor X pH 7.4, 37°C, mutant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
 Homo sapiens ?
-
 ?
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O i.e. chromozym t-PA Homo sapiens N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 Factor X pH 7.4, 37°C, wild-type enzyme Homo sapiens
10.2
-
 Factor X pH 7.4, 37°C, mutant enzyme Homo sapiens
12.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme Homo sapiens
24.6
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q Homo sapiens
49.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme, addition of tissue factor Homo sapiens
49.8
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor Homo sapiens