Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.21 extracted from

  • Persson, E.; Bak, H.; Olsen, O.H.
    Substitution of valine for leucine 305 in factor VIIa increases the intrinsic enzymatic activity (2001), J. Biol. Chem., 276, 29195-29199.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
tissue factor activates Homo sapiens

Protein Variants

Protein Variants Comment Organism
F374P the specific clotting activity in presence of tissue factor is 50% of that of the wild-type factor VIIa Homo sapiens
L305V mutant enzyme exhibits an increased rate of inhibition as compared with wild-type enzyme, both by D-Phe-Phe-Arg-chloromethyl ketone and antithombin III in presence of heparin. In complex with tissue factor both the amydolytic activity and the proteolytic activity are similar to that of the wild-type activity.The specific clotting activity in presence of tissue factor is 93% of that of the wild-type factor VIIa Homo sapiens
L305V/M306D/D309S amidylatic and proteolytic activity are virtually unaffected by the presence of tissue factor, 1.1fold increase. The specific clotting activity in presence of tissue factor is about 1% of that of the wild-type factor VIIa Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin III in presence of heparin Homo sapiens
D-Phe-Phe-Arg-chloromethyl ketone
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
Factor X wild-type enzyme, in absence of tissue factor Homo sapiens
0.0029
-
Factor X mutant enzyme L305V, in absence of tissue factor Homo sapiens
0.003
-
Factor X mutant enzyme L305V/M306D/D309S, in absence of tissue factor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.486
-
Factor X wild-type enzyme, in absence of tissue factor Homo sapiens
1.32
-
Factor X mutant enzyme L305V/M306D/D309S, in absence of tissue factor Homo sapiens
1.74
-
Factor X mutant enzyme L305V, in absence of tissue factor Homo sapiens