Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tissue factor | activates | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F374P | the specific clotting activity in presence of tissue factor is 50% of that of the wild-type factor VIIa | Homo sapiens |
L305V | mutant enzyme exhibits an increased rate of inhibition as compared with wild-type enzyme, both by D-Phe-Phe-Arg-chloromethyl ketone and antithombin III in presence of heparin. In complex with tissue factor both the amydolytic activity and the proteolytic activity are similar to that of the wild-type activity.The specific clotting activity in presence of tissue factor is 93% of that of the wild-type factor VIIa | Homo sapiens |
L305V/M306D/D309S | amidylatic and proteolytic activity are virtually unaffected by the presence of tissue factor, 1.1fold increase. The specific clotting activity in presence of tissue factor is about 1% of that of the wild-type factor VIIa | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin III | in presence of heparin | Homo sapiens | |
D-Phe-Phe-Arg-chloromethyl ketone | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
Factor X | wild-type enzyme, in absence of tissue factor | Homo sapiens | |
0.0029 | - |
Factor X | mutant enzyme L305V, in absence of tissue factor | Homo sapiens | |
0.003 | - |
Factor X | mutant enzyme L305V/M306D/D309S, in absence of tissue factor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.486 | - |
Factor X | wild-type enzyme, in absence of tissue factor | Homo sapiens | |
1.32 | - |
Factor X | mutant enzyme L305V/M306D/D309S, in absence of tissue factor | Homo sapiens | |
1.74 | - |
Factor X | mutant enzyme L305V, in absence of tissue factor | Homo sapiens |