Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tissue factor | factor VIIa remains in a zymogen-like state following proteolytic activation and depends on interactions with the cofactor tissue factor for function. Val21, Glu154 and Met156 cooperate to stabilize the conformation of the enzyme and to enhance the affinity for tissue factor | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V21N/E154I/M156Q | mutant enzyme with stabilized amino-terminal Ile16-Asp194 salt bridge and enhanced catalytic function | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-aminobenzamidine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, wild-type enzyme, with addition of tissue factor | Homo sapiens | |
0.9 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor | Homo sapiens | |
3.1 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor | Homo sapiens | |
5.5 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, wild-type enzyme, without tissue factor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | - |
Homo sapiens | ? | - |
? | |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O | i.e. chromozym t-PA | Homo sapiens | N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.4 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, wild-type enzyme, without tissue factor | Homo sapiens | |
32.2 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor | Homo sapiens | |
37.2 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, wild-type enzyme, with addition of tissue factor | Homo sapiens | |
47 | - |
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide | pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
p-aminobenzamidine | pH 8.0, wild-type enzyme, chromozym tPA as substrate with addition of tissue factor | Homo sapiens | |
0.124 | - |
p-aminobenzamidine | pH 8.0, mutant enzyme V21N/E154I/M156Q, chromozym tPA as substrate with addition of tissue factor | Homo sapiens | |
0.317 | - |
p-aminobenzamidine | pH 8.0, mutant enzyme V21N/E154I/M156Q, chromozym tPA as substrate without addition of tissue factor | Homo sapiens |