Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.21 extracted from

  • Petrovan, R.J.; Ruf, W.
    Role of zymogenicity-determining residues of coagulation factor VII/VIIa in cofactor interaction and macromolecular substrate recognition (2002), Biochemistry, 41, 9302-9309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
tissue factor factor VIIa remains in a zymogen-like state following proteolytic activation and depends on interactions with the cofactor tissue factor for function. Val21, Glu154 and Met156 cooperate to stabilize the conformation of the enzyme and to enhance the affinity for tissue factor Homo sapiens

Protein Variants

Protein Variants Comment Organism
V21N/E154I/M156Q mutant enzyme with stabilized amino-terminal Ile16-Asp194 salt bridge and enhanced catalytic function Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
p-aminobenzamidine
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, wild-type enzyme, with addition of tissue factor Homo sapiens
0.9
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor Homo sapiens
3.1
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor Homo sapiens
5.5
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, wild-type enzyme, without tissue factor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
Homo sapiens ?
-
?
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O i.e. chromozym t-PA Homo sapiens N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, wild-type enzyme, without tissue factor Homo sapiens
32.2
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor Homo sapiens
37.2
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, wild-type enzyme, with addition of tissue factor Homo sapiens
47
-
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.116
-
p-aminobenzamidine pH 8.0, wild-type enzyme, chromozym tPA as substrate with addition of tissue factor Homo sapiens
0.124
-
p-aminobenzamidine pH 8.0, mutant enzyme V21N/E154I/M156Q, chromozym tPA as substrate with addition of tissue factor Homo sapiens
0.317
-
p-aminobenzamidine pH 8.0, mutant enzyme V21N/E154I/M156Q, chromozym tPA as substrate without addition of tissue factor Homo sapiens