Activating Compound | Comment | Organism | Structure |
---|---|---|---|
prostasin | prostasin stimulates the conversion of the cell-associated 70-kDa matriptase zymogen to the two-chain active form. Prostasin induces matriptase zymogen activation in intestinal epithelial cells to regulate closure of the paracellular pathway. But active recombinant matriptase, however, does not require the expression of endogenous prostasin for barrier-forming activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA silencing of matriptase expression | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hepatocyte growth factor activator inhibitor 1 | HAI-1, a Kunitz-type inhibitor that functions both as a chaperone and a reversible inhibitor | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE | Homo sapiens |
70000 | - |
x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proform epithelial sodium channel + H2O | Homo sapiens | - |
mature epithelial sodium channel + ? | - |
? | |
proform G protein-coupled protease activated receptor-2 + H2O | Homo sapiens | - |
mature G protein-coupled protease activated receptor-2 + ? | - |
? | |
proform prostasin + H2O | Homo sapiens | - |
mature prostasin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the matriptase zymogen performs its own activation via autoproteolysis, also mutants S805A-matriptase and G827R-matriptase are readily cleaved to the two-chain form by recombinant matriptase | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 cell | - |
Homo sapiens | - |
epidermis | - |
Homo sapiens | - |
epithelium | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
skin | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
proform epithelial sodium channel + H2O | - |
Homo sapiens | mature epithelial sodium channel + ? | - |
? | |
proform G protein-coupled protease activated receptor-2 + H2O | - |
Homo sapiens | mature G protein-coupled protease activated receptor-2 + ? | - |
? | |
proform prostasin + H2O | - |
Homo sapiens | mature prostasin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | siRNA silencing of matriptase expression or inhibition of matriptase activity impairs development of transepithelial electrical resistance and increases the permeability of Caco-2 epithelial barriers | Homo sapiens |
metabolism | the matriptase zymogen is capable of intermolecular autoproteolytic activation, and the active enzyme is also an effective activator of the prostasin zymogen through direct proteolytic cleavage at its zymogen activation site. Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway. Activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates. Matriptase and not prostasin is the primary effector protease of tight junction assembly in simple columnar epithelia | Homo sapiens |
physiological function | matriptase promotes intestinal epithelial barrier formation | Homo sapiens |