Application | Comment | Organism |
---|---|---|
medicine | time-domain near infrared fluorescence (NIRF) imaging is applied to characterize expression and activity of matriptase in vivo in an orthotopic AsPC-1 pancreatic tumor model in nude mice. Strong expression and tumor-specific binding of intravenously injected antimatriptase antibody only to primary AsPC-1 tumors and their metastases is shown. Using a synthetic substrate it is shown that matriptase is proteolytically active in vitro as well as in vivo in tumor-bearing mice, and that application of synthetic active-site inhibitors can efficiently inhibit its proteolytic activity for at least 24 h | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
Western blot analysis, reducing conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MDA-MB-435 cell | RT-PCR analysis reveal a 1000fold higher matriptase expression in human pancreatic adenocarcinoma AsPC-1 cells than in the MDA-MB-435S cells | Homo sapiens | - |
PC-1 cell | RT-PCR analysis reveal a 1000fold higher matriptase expression in human pancreatic adenocarcinoma AsPC-1 cells than in the MDA-MB-435S cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(DY-681)-Gly-Arg-Gln-Ser-Arg-Ala-Ile-Lys (DY-681)-NH + H2O | synthetic substrate, peptide sequence is derived from one of the preferred matriptase cleavage sequences, P4(Arg/Lys)-P3(Xxx)-P2(Ser)-P1(Arg)-P10(Ala), where Xxx is a nonbasic amino acid | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
matriptase | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | RT-PACR analysis reveal a 1000fold higher matriptase expression in human pancreatic adenocarcinoma AsPC-1 cells than in the MDA-MB-435S cells | up |