Literature summary for 3.4.21.109 extracted from

Tsuzuki, S.; Miyake, Y.; Inouye, K.; Fushiki, T.
The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells (2009), Biosci. Biotechnol. Biochem., 73, 2538-2540.

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Cloned(Commentary)

Cloned (Comment)	Organism
full-length rat matriptase is expressed with a myc-epitope and a hexahistidine tag at its C-terminus in stably transfected Madin-Darby canine kidney cells	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
apical plasma membrane	matriptase C-terminal fragment molecules are delivered to both the apical and the basolateral side of Madin-Darby canine kidney cells	Rattus norvegicus	16324	-
basolateral plasma membrane	the N-terminal fragment part occurs on the basolateral side but poorly on the apical siden when expresssed in Madin-Darby canine kidney cells. Matriptase C-terminal fragment molecules are delivered to both the apical and the basolateral side of Madin-Darby canine kidney cells	Rattus norvegicus	16323	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	SDS-PAGE and Western blot, reducing conditions, proteolytically active, disulfide two-chain matriptase C-terminal fragment found in both the apical and basolateral media of stably transfected Madin-Darby canine kidney cells	Rattus norvegicus
93000	-	SDS-PAGE and Western blot, reducing conditions, proteolytically inactive, single-chain matriptase C-terminal fragment found in both the apical and basolateral media of stably transfected Madin-Darby canine kidney cells	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	matriptase is post-translationally processed via cleavage after Gly149. The N-terminal fragment part occurs on the basolateral side but poorly on the apical siden when expresssed in Madin-Darby canine kidney cells. Matriptase C-terminal fragment molecules are delivered to both the apical and the basolateral side of Madin-Darby canine kidney cells	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
matriptase	-	Rattus norvegicus

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Release 2023.1 (January 2023)