Cloned (Comment) | Organism |
---|---|
expressed in mammalian cells as a full-length rat matriptase with Myc-epitope-hexahistidine tag at its carboxyl-terminus | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus shows comparable kinetic activity to the full-length rat matriptase toward peptide substrate methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide acetate | Rattus norvegicus |
N772Q | Asn772 is N-glycosylated. Mutant N772Q consisting of the catalytic domain of matriptase bearing a N772Q mutation fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus is not detected in the medium conditioned by transfected cells but is on the cell surface and purified mutant N772Q exhibits markedly reduced activity toward peptide substrate methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide acetate | Rattus norvegicus |
S805A | mutant is unable to undergo activation when expressed in MDCK cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.164 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase bearing a N772Q mutation fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus | |
0.188 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus | |
0.2 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | full-length rat matriptase with Myc-epitope-hexahistidine tag at its carboxyl-terminus | Rattus norvegicus | |
0.228 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase and glycopeptidase F | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE, reducing condition, protease inhibitor cocktail, Western blotting using an anti-myc antibody in a sample of medium, two bands 93 kDa and 33 kDa are visible, 33 kDa fragment is a cleavage product of the carboxyl-terminal part | Rattus norvegicus |
43000 | - |
under non-reducing conditions in a sample of medium three bands 61 kDa, 55 kDa, 43 kDa are visible using Western blotting with a anti-myc antibody | Rattus norvegicus |
55000 | - |
under non-reducing conditions in a sample of medium three bands 61 kDa, 55 kDa, 43 kDa are visible using Western blotting with a anti-myc antibody | Rattus norvegicus |
61000 | - |
under non-reducing conditions in a sample of medium three bands 61 kDa, 55 kDa, 43 kDa are visible using Western blotting with a anti-myc antibody | Rattus norvegicus |
93000 | - |
SDS-PAGE, reducing condition, protease inhibitor cocktail, Western blotting using an anti-myc antibody in a sample of medium, two bands 93 kDa and 33 kDa are visible. 93 kDa fragment represents released single-chain carboxyl-terminal part | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | catalytic domain is N-glycosylated at Asn772 | Rattus norvegicus |
proteolytic modification | matriptase is post-translationally processed by cleavage with unknown proteases between Gly149 and Ser150 within the sea-urchin sperm protein-enterokinase-agrin (SEA) domain | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
purified from the conditioned medium using Ni-NTA chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide + H2O | - |
Rattus norvegicus | methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
matriptase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase bearing a N772Q mutation fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus | |
59 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | full-length rat matriptase with Myc-epitope-hexahistidine tag at its carboxyl-terminus | Rattus norvegicus | |
91 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus | |
103 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase and glycopeptidase F | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.45 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase bearing a N772Q mutation fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus | |
290 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | full-length rat matriptase with Myc-epitope-hexahistidine tag at its carboxyl-terminus | Rattus norvegicus | |
454 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase and glycopeptidase F | Rattus norvegicus | |
484 | - |
methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide | construct consisting of the catalytic domain of matriptase fused to Myc-epitope-hexahistidine tag at its carboxyl-terminus treated with recombinant enterokinase | Rattus norvegicus |