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Literature summary for 3.4.21.109 extracted from
- Inhibition of human matriptase by eglin c variants (2006), FEBS Lett., 580, 2227-2232.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged amino acids 596-855 of matriptase in Escherichia coli strain M15 | Homo sapiens |
| into pQE-vecto and expressed in Escherichia coli M15 strain | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | wild type eglin c has no effect | Homo sapiens | |
| R1K'4-eglin | different eglin c variants with differing inhibitory potential versus matriptase, construction, expression and purification of eglin c variants and screening for inhibitory potency, overview, R1K'4-eglin has the wild-type Pro45 at P1 position and Tyr49 at P4' position residues replaced with Arg and Lys, respectively, leads to the production of a selective, high affinity and proteolytically stable inhibitor of matriptase, molecular modeling of enzyme-inhibitor complex, overview | Homo sapiens |  |
| R1K4-eglin | wild type eglin c with Pro45 at P1 position and Tyr49 residues at P4 position replaced with Arg and Lys respectively, most potent, selective, high affinity and proteolytically stable inhibitor | Homo sapiens | |
| R4R1-eglin | with substituted P42 and L45 for arginine residues, variants containing an Arg or Lys at position 49 instead of the original Tyr residue show enhanced inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | matriptase is a type II transmembrane serine protease | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
refolded zymogen | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by immobilized metal-chelate affinity chromatography and ion exchange chromatography | Homo sapiens |
| recombinant His-tagged amino acids 596-855 of matriptase from Escherichia coli strain M15 by metal affinity chromatography, dialysis, and anion exchange chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epithelial cancer cell | overexpression of the enzyme | Homo sapiens | - |
| epithelium | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Boc-Glu-Ala-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Boc-Glu-Ala-Arg + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs ot the type II transmembrane serine protease family | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Homo sapiens |
| 9 | 11 | purified enzyme | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000004 | - |
R1K4-eglin | - |
Homo sapiens | |
| 0.000004 | - |
R1K'4-eglin | pH 9.0, recombinant His-tagged amino acids 596-855 of matriptase | Homo sapiens | |
| 0.000026 | - |
R4R1-eglin | - |
Homo sapiens | |
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