Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 C43(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystals of the truncated GlpG wild type enzyme are obtained by mixing 2.5-3 M ammonium chloride with protein at ratio of 1:1 in hanging drops at 25°C | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A253I | the mutant exhibits 16% of wild type activity towards the wild type TatA protein but shows 87% of wild type activity on TatA mutant A8G | Escherichia coli |
A253L | the mutant exhibits no activity of wild type activity towards the wild type TatA protein but shows 23% of wild type activity on TatA mutant A8G | Escherichia coli |
A253T | the mutant exhibits 37% of wild type activity towards the wild type TatA protein but shows 63% of wild type activity on TatA mutant A8G | Escherichia coli |
A253V | the mutant exhibits 63% of wild type activity towards the wild type TatA protein but shows 144% of wild type activity on TatA mutant | Escherichia coli |
H150A | the mutation leads to a complete loss of activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-amino-4-chloro-3-methoxyisocoumarin | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gurken protein + H2O | Escherichia coli | - |
? | - |
? | |
TatA protein + H2O | Escherichia coli | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09391 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Mono-Q column chromatography, and Superdex-200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gurken protein + H2O | - |
Escherichia coli | ? | - |
? | |
TatA protein + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlpG | - |
Escherichia coli |
rhomboid protease | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.006 | - |
in 50 mM HEPES-NaOH (pH 7.5), 0.4 M NaCl, 5 mM EDTA, 10% (v/v) glycerol, and 0.05% (w/v) n-dodecyl-beta-D-maltoside, at 37°C | Escherichia coli | 7-amino-4-chloro-3-methoxyisocoumarin |