Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-fusion protein | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
D256A/R257A | Golgi localisation is not affected in the mutant protein | Toxoplasma gondii |
F36A/F37A | mutant is still targeted efficiently to the Golgi compartment, indicating that in TgROM2 there are two crucial signal elements responsible for Golgi targeting | Toxoplasma gondii |
F36A/F37A/D256A/R257A | Golgi localisation is not affected in the mutant protein | Toxoplasma gondii |
F54A/P55S/H56A/F57A | micronemes localisation is abolished | Toxoplasma gondii |
additional information | by analyzing chimeric proteins it is shown that the N-terminal domain of TgROM2 is sufficient to confer Golgi localisation to related ROM proteins that are normally localised to the plasma membrane or to micronemes. F36 and F37 are crucial in this targeting process | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Toxoplasma gondii | 5794 | - |
microneme | N-terminal domain is responsible for subcellular localisation of TgROM1, in particular the sequence FPHF is essential (amino acids 54-57) | Toxoplasma gondii | 20009 | - |
plasma membrane | - |
Toxoplasma gondii | 5886 | - |
plasma membrane | analyzed using indirect immunofluorescence assays | Toxoplasma gondii | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography on glutathione agarose | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole body | - |
Toxoplasma gondii | - |
Synonyms | Comment | Organism |
---|---|---|
rhomboid protease | - |
Toxoplasma gondii |
TgROM1 | - |
Toxoplasma gondii |
TgROM2 | - |
Toxoplasma gondii |
TgROM4 | - |
Toxoplasma gondii |
TgROM5 | - |
Toxoplasma gondii |