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Literature summary for 3.4.21.105 extracted from
- Microneme rhomboid protease TgROM1 is required for efficient intracellular growth of Toxoplasma gondii (2008), Eukaryot. Cell, 7, 664-674.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type HA9-tagged ROM1 and of muant ROM1 in the parasites, repression of Tet-regulatable HA9-ROM1 expression in the presence of Atc, overview | Toxoplasma gondii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of ROM1-knockout mutant, ROM1-deficient parasites are outcompeted by wild-type Toxoplasma gondii, the ROM1-deficient parasites show only modest decrease in invasion but replicate more slowly than wild-type cells, overview | Toxoplasma gondii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | ROM1 | Toxoplasma gondii | 5794 | - |
| membrane | Toxoplasma gondii contains six rhomboids that are expressed in different life cycle stages and localized to different cellular compartments, ROM1 is not released onto the parasite surface during cell invasion, subcellular localization of HA9-tagged ROM1 in intracellular parasites, overview | Toxoplasma gondii | 16020 | - |
| microneme | ROM1 | Toxoplasma gondii | 20009 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Toxoplasma gondii | ROM1 does not play a critical role in cell invasion, ROM1-deficient parasites are outcompeted by wild-type Toxoplasma gondii, the ROM1-deficient parasites show only modest decrease in invasion but replicate more slowly than wild-type cells, ROM1 is required for efficient intracellular growth of the parasite, overview | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | Q695U0 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains | ROM1 of Toxoplasma gondii contains a catalytic triad consisting of asparagine, histidine, and serine residues of in transmembrane domains 2, 6, and 4 | Toxoplasma gondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Toxoplasma gondii contains six rhomboids that are expressed in different life cycle stages and localized to different cellular compartments | Toxoplasma gondii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ROM1 does not play a critical role in cell invasion, ROM1-deficient parasites are outcompeted by wild-type Toxoplasma gondii, the ROM1-deficient parasites show only modest decrease in invasion but replicate more slowly than wild-type cells, ROM1 is required for efficient intracellular growth of the parasite, overview | Toxoplasma gondii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| microneme rhomboid protease | - |
Toxoplasma gondii |
| rhomboid protein 1 | - |
Toxoplasma gondii |
| ROM1 | - |
Toxoplasma gondii |
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