Application | Comment | Organism |
---|---|---|
analysis | application to N-terminal pyroglutamyl unblocking prior to Edman sequential degradation in peptide and protein sequencing | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.04 | - |
Pyroglutamyl-beta-naphthylamide | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25200 | - |
4 * 25200, SDS-PAGE | Bacillus subtilis |
91000 | - |
non-denaturing PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.2 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 70% loss of activity after 1 month | Bacillus subtilis |
4°C, 50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 13% loss of activity after 1 month | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyroglutamyl-beta-naphthylamide + H2O | - |
Bacillus subtilis | pyroglutamate + beta-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25200, SDS-PAGE | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month | Bacillus subtilis |