Literature summary for 3.4.19.12 extracted from

Susor, A.; Liskova, L.; Toralova, T.; Pavlok, A.; Pivonkova, K.; Karabinova, P.; Lopatarova, M.; Sutovsky, P.; Kubelka, M.
Role of ubiquitin C-terminal hydrolase-L1 in antipolyspermy defense of mammalian oocytes (2010), Biol. Reprod., 82, 1151-1161.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of EGFP-tagged UCHL1 in oocytes	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
3-amino-2-(4-methylbenzoyl)-6-oxo-6,7-dihydrothieno[2,3-b]pyridine-5-carboxylaic acid	an uncompetitive inhibitor of UCHL1 that binds only to the Michaelis complex and not to free enzyme	Bos taurus
LDN-57444	a reversible, competitive, active site-directed isatin oxime with consistent preference for UCHL1 over UCHL3 by 28fold	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	in oocytes	Bos taurus	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	x * 27000, SDS-PAGE	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
oocyte	subcortical localization of native UCHL1 in bovine oocytes during maturation. Expression of UCHL1 monomer does not change during meiotic maturation of bovine oocytes. Developmental competence is unaffected in parthenogenetic embryos that originated from UCH-L1 inhibitor treated oocytes	Bos taurus	-

Subunits

Subunits	Comment	Organism
?	x * 27000, SDS-PAGE	Bos taurus
More	presumptive oligomeric forms of UCHL1 increase in the presence of UCHL1 inhibitors	Bos taurus

Synonyms

Synonyms	Comment	Organism
ubiquitin C-terminal hydrolase-L1	-	Bos taurus
UCHL1	-	Bos taurus

General Information

General Information	Comment	Organism
additional information	activity of UCHL1 stimulates ligase activity of this enzyme in bovine oocytes, resulting in increased protein stabilization or modification through K63-linked polyubiquitination	Bos taurus
physiological function	UCHL1 promotes disassembly of polyubiquitin chains during meiotic maturation. Conversely, UCHL1 regulates formation of polyubiquitin chains in bovine oocytes	Bos taurus

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Release 2023.1 (January 2023)