Cloned (Comment) | Organism |
---|---|
ataxin-3 carrying six consecutive glutamines cloned into plasmid pGEX6P-1 and expressed as a GST fusion protein in the Escherichia coli Codon Plus-RIL strain | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C14A | mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature | Mus musculus |
C14A/H119L | mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature | Mus musculus |
H119L | mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA, HgCl2 and p-chloro-mecuribenzoate do not inhibit fragmentation of AT-3 carrying six consecutive glutamines | Mus musculus | |
tosyl-L-phenylalanine chloromethyl ketone | inhibits fragmentation of AT-3 carrying six consecutive glutamines | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9CVD2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AT-3 carrying six consecutive glutamines undergoes fragmentation upon incubation at room temperature. Cys14 and His119 are involved in the autolytic activity | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AT-3 | - |
Mus musculus |
ataxin-3 | - |
Mus musculus |