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Literature summary for 3.4.19.12 extracted from

  • Mauri, P.L.; Riva, M.; Ambu, D.; De Palma, A.; Secundo, F.; Benazzi, L.; Valtorta, M.; Tortora, P.; Fusi, P.
    Ataxin-3 is subject to autolytic cleavage (2006), FEBS J., 273, 4277-4286.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ataxin-3 carrying six consecutive glutamines cloned into plasmid pGEX6P-1 and expressed as a GST fusion protein in the Escherichia coli Codon Plus-RIL strain Mus musculus

Protein Variants

Protein Variants Comment Organism
C14A mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature Mus musculus
C14A/H119L mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature Mus musculus
H119L mutant of ataxin-3 carrying six consecutive glutamines, does not undergo proteolytic fragmentation on incubation at room temperature Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA, HgCl2 and p-chloro-mecuribenzoate do not inhibit fragmentation of AT-3 carrying six consecutive glutamines Mus musculus
tosyl-L-phenylalanine chloromethyl ketone inhibits fragmentation of AT-3 carrying six consecutive glutamines Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9CVD2
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information AT-3 carrying six consecutive glutamines undergoes fragmentation upon incubation at room temperature. Cys14 and His119 are involved in the autolytic activity Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
AT-3
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Mus musculus
ataxin-3
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Mus musculus