Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 6 h, 1 mM, 90% inhibition | Patiria pectinifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 1.7fold at 25°C and pH 7.5 | Patiria pectinifera | |
Zn2+ | 1 mol Zn2+ per mol of enzyme | Patiria pectinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Patiria pectinifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Patiria pectinifera | - |
starfish | - |
Purification (Comment) | Organism |
---|---|
native enzyme 418fold from pyloric ceca to near homogeneity by gel filtration, anion exchange chromatography, and another step of gel filtration | Patiria pectinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pyloric cecum | - |
Patiria pectinifera | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Patiria pectinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Gly-L-Arg + H2O | - |
Patiria pectinifera | benzoyl-Gly + L-Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Patiria pectinifera |
Synonyms | Comment | Organism |
---|---|---|
CPB | - |
Patiria pectinifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Patiria pectinifera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | inactivation above 50°C | Patiria pectinifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | 15 min, purified enzyme, stable | Patiria pectinifera |
50 | - |
15 min, the purified enzyme is unstable above | Patiria pectinifera |
60 | - |
15 min, loss of 70% of purified enzyme activity | Patiria pectinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Patiria pectinifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | high loss of activity below pH 6.0 and above pH 10.0 | Patiria pectinifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
30 min, inactivation | Patiria pectinifera |
5 | - |
the purified enzyme is unstable below | Patiria pectinifera |
6 | 10 | purified enzyme, stable | Patiria pectinifera |