Literature summary for 3.4.17.2 extracted from

Adler, M.; Bryant, J.; Buckman, B.; Islam, I.; Larsen, B.; Finster, S.; Kent, L.; May, K.; Mohan, R.; Yuan, S.; Whitlow, M.
Crystal structures of potent thiol-based inhibitors bound to carboxypeptidase B (2005), Biochemistry, 44, 9339-9347.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with inhibitors DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid, (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid, or 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid, hanging drop vapour diffusion method, 0.21 mg/ml protein in 10 mM Tris, pH 7.0, 1 mM inhibitor, and 50 mM NaCl, mixed with an equal volume of 0.002 ml of reservoir solution containing 0.15-0.30 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 12-22% PEG 8000, X-ray diffraction structure determination and analysis at 1.4-2.0 A resolution	Sus scrofa

Crystallization (Comment)

Organism

enzyme in complex with inhibitors DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid, (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid, or 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid, hanging drop vapour diffusion method, 0.21 mg/ml protein in 10 mM Tris, pH 7.0, 1 mM inhibitor, and 50 mM NaCl, mixed with an equal volume of 0.002 ml of reservoir solution containing 0.15-0.30 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 12-22% PEG 8000, X-ray diffraction structure determination and analysis at 1.4-2.0 A resolution

Sus scrofa

Inhibitors

Inhibitors	Comment	Organism
(2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid	binding structure analysis	Sus scrofa
2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid	binding structure analysis	Sus scrofa
5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid	binding structure analysis	Sus scrofa
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid	binding structure analysis	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P09955	-	-

Purification (Commentary)

Purification (Comment)	Organism
further purification of the commercial pancreatic preparation by hydrophobic interaction chromatography	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Sus scrofa	-
pancreas	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuryl-L-arginine + H2O	-	Sus scrofa	hippuric acid + L-Arg	-	?

Synonyms

Synonyms	Comment	Organism
pp-CpB	-	Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Sus scrofa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Sus scrofa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.000002	-	(2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid	pH 7.4, 22°C	Sus scrofa
0.000002	-	5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid	pH 7.4, 22°C	Sus scrofa
0.000007	-	2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid	pH 7.4, 22°C	Sus scrofa
0.000063	-	DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid	pH 7.4, 22°C	Sus scrofa