Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitors DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid, 2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid, (2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid, or 5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid, hanging drop vapour diffusion method, 0.21 mg/ml protein in 10 mM Tris, pH 7.0, 1 mM inhibitor, and 50 mM NaCl, mixed with an equal volume of 0.002 ml of reservoir solution containing 0.15-0.30 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 12-22% PEG 8000, X-ray diffraction structure determination and analysis at 1.4-2.0 A resolution | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid | binding structure analysis | Sus scrofa | |
2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid | binding structure analysis | Sus scrofa | |
5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid | binding structure analysis | Sus scrofa | |
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | binding structure analysis | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P09955 | - |
- |
Purification (Comment) | Organism |
---|---|
further purification of the commercial pancreatic preparation by hydrophobic interaction chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Sus scrofa | - |
pancreas | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-L-arginine + H2O | - |
Sus scrofa | hippuric acid + L-Arg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pp-CpB | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
(2R)-2-(3-carbamimidamidophenyl)-3-sulfanylpropanoic acid | pH 7.4, 22°C | Sus scrofa | |
0.000002 | - |
5-carbamimidamido-2-(sulfanylmethyl)pentanoic acid | pH 7.4, 22°C | Sus scrofa | |
0.000007 | - |
2-(5-carbamimidamido-2-chlorophenyl)-3-sulfanylpropanoic acid | pH 7.4, 22°C | Sus scrofa | |
0.000063 | - |
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | pH 7.4, 22°C | Sus scrofa |