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Literature summary for 3.4.17.2 extracted from
- Preparation, characterization, and application of a novel immobilized carboxypeptidase B (1989), Appl. Biochem. Biotechnol., 22, 31-43.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the immobilized carboxypeptidase B is used for stepwise digestion of cytochrome c | Sus scrofa |
General Stability
| General Stability | Organism |
|---|---|
| heat stability is markedly increased as an effect of immobilization | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
hippuryl-L-Arg | soluble enzyme | Sus scrofa |  |
| 28 | - |
hippuryl-L-Arg | immobilized enzyme | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20-25°C, stable for at least 1 year, immobilized enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hippuryl-L-Arg | - |
Sus scrofa | hippuric acid + L-Arg | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
soluble enzyme | Sus scrofa |
| 55 | - |
immobilized enzyme | Sus scrofa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: 65% of maximal activity, 60°C: 50% of maximal activity | Sus scrofa |
| 35 | 65 | 35°C: 45% of maximal activity, 65°C: about 50% of maximal activity, immobilized enzyme | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat stability is markedly increased as an effect of immobilization | Sus scrofa |
| 20 | 25 | stable for at least 1 year, immobilized enzyme | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 7.7 | soluble and immobilized enzyme | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about 40% of maximal activity at pH 6 and 9, soluble enzyme and immobilized enzyme | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH 4.5 is the optimal pH for immobilization | Sus scrofa |
| 6.7 | 8.8 | 60°C, optimal stability of the immobilized enzyme | Sus scrofa |
| 7.5 | - |
60°C, optimal stability of the soluble enzyme | Sus scrofa |
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