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Literature summary for 3.4.17.1 extracted from

  • Abrunhosa, L.; Venancio, A.
    Isolation and purification of an enzyme hydrolyzing ochratoxin A from Aspergillus niger (2007), Biotechnol. Lett., 29, 1909-1914.
    View publication on PubMed

Application

Application Comment Organism
food industry the application of the novel ochratoxin A hydrolytic enzyme to reduce the ochratoxin A contents on some food or feed products is under evaluation Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
ochratoxin A
-
Aspergillus niger
1.1
-
ochratoxin A
-
Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the enzyme is purified from the culture medium by acetone precipitation and Macro-Prep High Q anion exchange chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
carboxypeptidase A, pH 5.6 Aspergillus niger
2.8
-
Aspergillus niger extract, pH 5.6 Aspergillus niger
2.8
-
carboxypeptidase A, pH 7.5 Aspergillus niger
9.2
-
Aspergillus niger extract, pH 8.5 Aspergillus niger
10.8
-
carboxypeptidase A, pH 8.5 Aspergillus niger
14.4
-
culture filtrate Aspergillus niger
33.5
-
Aspergillus niger extract, pH 7.5 Aspergillus niger
33.5
-
purification step acetone precipitation Aspergillus niger
35.8
-
purification step Macro-Prep High Q Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ochratoxin A + H2O
-
Aspergillus niger ochratoxin alpha + L-beta-phenylalanine
-
?

Synonyms

Synonyms Comment Organism
carboxypeptidase a
-
Aspergillus niger
ochratoxin A hydrolytic enzyme
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
activity assay Aspergillus niger