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Literature summary for 3.4.17.1 extracted from

  • Lee, K.J.; Kim, D.H.
    Design of mechanism-based carboxypeptidase A inactivators on the basis of the X-ray crystal structure and catalytic reaction pathway (1998), Bioorg. Med. Chem., 6, 1613-1622.
    View publication on PubMed
Search for more literature for 3.4.17.1

Application

Application Comment Organism
medicine design of mechanism-based, specific inhibitors to cure disease caused by enzyme overfunction Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
structural analysis of enzyme-GlyTyr complexes, design of mechanism-based inactivators based thereon Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
N-(hydroxyacetyl)-Phe irreversible inactivation, Kinact/Ki: 71 M-1s-1 Bos taurus
O-(hydroxyacetyl)-beta-phenyllactate irreversible inactivation, Kinact/Ki: 57 M-1s-1 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro mechanism Bos taurus
a
release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro evidence of general base pathway Bos taurus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Tyr + H2O low activity Bos taurus Gly + L-Tyr
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bos taurus