General Stability | Organism |
---|---|
generally unstable at all stages of purification | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Rattus norvegicus | |
3-Phenylpropionic acid | Ki: 0.81 mM, noncompetitive | Rattus norvegicus | |
Chelating agents | - |
Rattus norvegicus | |
Hydroxyquinoline sulfonate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
hippuryl-L-phenyllactate | - |
Rattus norvegicus | |
2.7 | - |
hippuryl-L-Phe | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloenzyme | Rattus norvegicus | |
Zn2+ | 1 mol Zn per mol of enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
SDS-PAGE, component II | Rattus norvegicus |
39300 | - |
SDS-PAGE, component I | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 components: I, II | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | mast cell | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, NaCl-HEPES, pH 7.5, purified enzyme, 1 month, 10% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-DL-phenyllactate + H2O | - |
Rattus norvegicus | hippuric acid + DL-phenyllactate | - |
ir | |
hippuryl-L-Phe + H2O | - |
Rattus norvegicus | hippuric acid + L-Phe | - |
ir | |
hippuryl-L-phenyllactate + H2O | - |
Rattus norvegicus | hippuric acid + L-phenyllactate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min, 50% loss of activity | Rattus norvegicus |
50 | - |
10 min, 50% loss of activity | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
hippuryl-L-Phe | - |
Rattus norvegicus | |
933 | - |
hippuryl-L-phenyllactate | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Rattus norvegicus |
7.5 | - |
assay at | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5: about 20% of maximum activity, pH 10: about 50% of maximum activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Rattus norvegicus |