Any feedback?
Literature summary for 3.4.16.5 extracted from
- Purification and characterization of a new type of serine carboxypeptidase from Monascus purpureus (2004), J. Ind. Microbiol. Biotechnol., 31, 23-28.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 10% inhibition | Monascus purpureus |  |
| CaCl2 | 1 mM, 16% inhibition | Monascus purpureus | |
| chymostatin | 1 mM, 89% inhibition | Monascus purpureus | |
| CuCl | 1 mM, 37% inhibition | Monascus purpureus | |
| CuCl2 | 1 mM, 39% inhibition | Monascus purpureus | |
| diisopropylfluorophosphate | 1 mM, 63% inhibition | Monascus purpureus | |
| FeCl3 | 1 mM, 92% inhibition | Monascus purpureus | |
| HgCl2 | 1 mM, 54% inhibition | Monascus purpureus | |
| MnCl2 | 1 mM, 14% inhibition | Monascus purpureus | |
| Nalpha-tosyl-L-Lys-chloromethyl ketone | 1 mM, 13% inhibition | Monascus purpureus | |
| Nalpha-tosyl-L-Phe-chloromethyl ketone | 1 mM, 57% inhibition | Monascus purpureus | |
| NEM | 1 mM, 71% inhibition | Monascus purpureus | |
| PbCl2 | 1 mM, 95% inhibition | Monascus purpureus | |
| PCMB | 1 mM, 95% inhibition | Monascus purpureus | |
| pepstatin A | 1 mM, 8% inhibition | Monascus purpureus | |
| Phenylmethylsulfonylfluoride | 1 mM, 72% inhibition | Monascus purpureus | |
| piperastatin A | 1 mM, 70% inhibition | Monascus purpureus | |
| SDS | 1 mM, complete inhibition | Monascus purpureus | |
| SnCl2 | 1 mM, complete inhibition | Monascus purpureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.86 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C | Monascus purpureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
| 67000 | - |
1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
| 132000 | - |
gel filtration | Monascus purpureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monascus purpureus | - |
IFO 4478 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 17% carbohydrate | Monascus purpureus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Monascus purpureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.13 | - |
- |
Monascus purpureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Ala-Glu + H2O | 76% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Ala + Glu | - |
? | |
| benzyloxycarbonyl-Glu-Phe + H2O | 119% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Glu + Phe | - |
? | |
| Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Monascus purpureus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | 59% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Gly-Pro-Leu + Gly | - |
? | |
| benzyloxycarbonyl-Phe-Ala + H2O | 77% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Ala | - |
? | |
| benzyloxycarbonyl-Phe-Gly + H2O | 24% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Gly | - |
? | |
| benzyloxycarbonyl-Phe-Leu + H2O | 21% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Phe + Leu | - |
? | |
| Benzyloxycarbonyl-Phe-Tyr + H2O | 18% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | Benzyloxycarbonyl-Phe + Tyr | - |
? | |
| benzyloxycarbonyl-Tyr-Glu + H2O | 134% of the activity with benzyloxycarbonyl-Glu-Tyr | Monascus purpureus | benzyloxycarbonyl-Tyr + Glu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 64000 + 1 * 67000, SDS-PAGE | Monascus purpureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
hydrolysis of benzyloxycarbonyl-Glu-Tyr | Monascus purpureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 5.0, 15 min, stable up to | Monascus purpureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 291 | - |
benzyloxycarbonyl-Tyr-Glu | pH 4.0, 37°C | Monascus purpureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
hydrolysis of benzyloxycarbonyl-Glu-Tyr | Monascus purpureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | 37°C, 1 h, stable | Monascus purpureus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Monascus purpureus | - |
- |
3.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
