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Literature summary for 3.4.16.5 extracted from

  • Barth, R.; Wolf, D.H.; Holzer, H.
    Studies on the carboxypeptidase Y-inhibitor complex of yeast (1978), Biochim. Biophys. Acta, 527, 63-69.
    View publication on PubMed
Search for more literature for 3.4.16.5

Protein Variants

Protein Variants Comment Organism
L267D/L272A kcat/Km of mutant enzyme in% of the of the wild-type value: 1.2% for furylacryloyl-Ala-Leu, 13% for furylacryloyl-Ala-Glu, 423% for furylacryloyl-Ala-Lys, 70% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
L272D kcat/Km of mutant enzyme in% of the of the wild-type value: 10.4% for furylacryloyl-Ala-Leu, 67% for furylacryloyl-Ala-Glu, 61.9% for furylacryloyl-Ala-Lys, 26% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
L272E kcat/Km of mutant enzyme in% of the of the wild-type value: 4% for furylacryloyl-Ala-Leu, 30% for furylacryloyl-Ala-Glu, 257% for furylacryloyl-Ala-Lys, 70% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
L272Q kcat/Km of mutant enzyme in% of the of the wild-type value: 3.3% for furylacryloyl-Ala-Leu, 70% for furylacryloyl-Ala-Glu, 87% for furylacryloyl-Ala-Lys, 32% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
L272R/M398F kcat/Km of mutant enzyme in% of the of the wild-type value: 6.3% for furylacryloyl-Ala-Leu, 176% for furylacryloyl-Ala-Glu, 0.3% for furylacryloyl-Ala-Lys, 0.2% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
L272R/T60F kcat/Km of mutant enzyme in% of the of the wild-type value: 60% for furylacryloyl-Ala-Leu, 172% for furylacryloyl-Ala-Glu, 2.8% for furylacryloyl-Ala-Lys, 2.1% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
S297D kcat/Km of mutant enzyme in% of the of the wild-type value: 39.6% for furylacryloyl-Ala-Leu, 72% for furylacryloyl-Ala-Glu, 73% for furylacryloyl-Ala-Lys, 25% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
S297E kcat/Km of mutant enzyme in% of the of the wild-type value: 27% for furylacryloyl-Ala-Leu, 63% for furylacryloyl-Ala-Glu, 58% for furylacryloyl-Ala-Lys, 24% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
S297Q kcat/Km of mutant enzyme in% of the of the wild-type value: 25% for furylacryloyl-Ala-Leu, 80% for furylacryloyl-Ala-Glu, 41% for furylacryloyl-Ala-Lys, 19.3% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae
T60F/L267D/L272A kcat/Km of mutant enzyme in% of the of the wild­type value: 6% for furylacryloyl-Ala-Leu, 80% for furylacryloyl-Ala-Glu, 789% for furylacryloyl-Ala-Lys, 280% for furylacryloyl-Ala-Arg Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information studies on the carboxypeptidase Y-inhibitor complex of yeast Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzyloxycarbonyl-Gly-Phe + H2O
-
 Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Phe
-
 ?
benzyloxycarbonyl-Leu-Leu + H2O
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 Saccharomyces cerevisiae benzyloxycarbonyl-Leu + Leu
-
 ?
benzyloxycarbonyl-Leu-Phe + H2O
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 Saccharomyces cerevisiae benzyloxycarbonyl-Leu + Phe
-
 ?
benzyloxycarbonyl-Phe-Leu + H2O
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 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
-
 ?