Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.16.5 extracted from

  • Zuber, H.
    Carboxypeptidase C (1976), Methods Enzymol., 45, 561-568.
    View publication on PubMed
Search for more literature for 3.4.16.5

General Stability

General Stability Organism
orange peel enzyme is inactivated upon lyophilization Citrus sp.

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate peel enzyme inhibited, leaf enzyme not Citrus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 benzyloxycarbonyl-Leu-Phe orange peel Citrus sp.
4
-
 benzyloxycarbonyl-Arg-Pro orange peel Citrus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc possibly contains zinc in the active site, orange peel Citrus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 enzyme from peel, gel filtration Citrus sp.
175000
-
 enzyme from leaf, gel filtration Citrus sp.

Organism

Organism UniProt Comment Textmining
Citrus sp.
-
-
-
Citrus sp.
-
 orange
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrus sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Citrus sp.
-
peel flavedo Citrus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Citrus sp.

Storage Stability

Storage Stability Organism
-20°C, pH 4.7-5.5, stable for several months Citrus sp.
4°C, pH 4.7-5.5, stable for a few days Citrus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Pro + H2O
-
 Citrus sp. benzyloxycarbonyl-Arg + Pro
-
 ?
benzyloxycarbonyl-Leu-Phe + H2O
-
 Citrus sp. benzyloxycarbonyl-Leu + Phe
-
 ?
additional information splits off most readily aromatic and aliphatic amino acid residues at the carboxyl end Citrus sp. ?
-
 ?
additional information glycine is split off very slowly Citrus sp. ?
-
 ?
additional information neutral, basic and acid amino acids including proline are all hydrolyzed to approximately the same extent Citrus sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3 5.7 benzyloxycarbonyl-Leu-Phe, benzyloxycarbonyl-Arg-Pro, orange peel Citrus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 orange leaf enzyme is unstable below pH 4 and above pH 6 Citrus sp.
4 6 stable, irreversible inactivation above pH 6, orange peel Citrus sp.
5
-
 most stable at, orange leaf enzyme Citrus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Citrus sp. orange leaf
-
 4.5