Any feedback?
Literature summary for 3.4.15.1 extracted from
- Expression and characterization of recombinant human angiotensin I-converting enzyme. Evidence for a C-terminal transmembrane anchor and for a proteolytic processing of the secreted recombinant and plasma enzymes (1991), J. Biol. Chem., 266, 5540-5546.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | activates | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Chinese hamster ovary cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| captopril | - |
Homo sapiens | |
| EDTA | recombinant enzyme, restored by ZnSO4 | Homo sapiens | |
| enaprilat | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
angiotensin I | recombinant enzyme | Homo sapiens | |
| 0.136 | - |
2-furanacryloyl-Phe-Gly-Gly | wild-type enzyme | Homo sapiens | |
| 0.174 | - |
2-furanacryloyl-Phe-Gly-Gly | recombinant enzyme | Homo sapiens | |
| 1.54 | - |
hippuryl-His-Leu | recombinant enzyme | Homo sapiens | |
| 1.61 | - |
hippuryl-His-Leu | wild-type enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | anchored to the plasma membrane by the C-terminal transmembrane domain | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
x * 170000, kidney enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the secreted form is derived from the membrane-bound form by a post-translational proteolytic cleavage of the C-terminal region | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-furanacryloyl-Phe-Gly-Gly + H2O | - |
Homo sapiens | 2-furanacryloyl-Phe + Gly-Gly | - |
? | |
| angiotensin I + H2O | - |
Homo sapiens | angiotensin II + His-Leu | - |
? | |
| hippuryl-His-Leu + H2O | - |
Homo sapiens | hippuric acid + His-Leu | - |
? | |
| Substance P + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 170000, kidney enzyme, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.3 | - |
Substance P | free acid, recombinant enzyme, and wild-type enzyme | Homo sapiens | |
| 320 | - |
2-furanacryloyl-Phe-Gly-Gly | recombinant enzyme | Homo sapiens | |
| 368 | - |
2-furanacryloyl-Phe-Gly-Gly | wild-type enzyme | Homo sapiens | |
| 408 | - |
hippuryl-His-Leu | recombinant enzyme | Homo sapiens | |
| 414 | - |
hippuryl-His-Leu | wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
recombinant and wild-type enzyme | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | pH 7.5: about 40% of maximal activity, pH 9.0: about 75% of maximal activity | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
