Cloned (Comment) | Organism |
---|---|
regions of the testis ACE sequence are replaced with the corresponding N-domain sequence. The resultant chimeras C1-163Ndom-ACE, C417-579Ndom-ACE, and C583-623Ndom-ACE are processed to the cell surface of transfected Chinese hamster ovary cells, and are cleaved at the identical site as that of tACE | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | regions of the testis ACE sequence are replaced with the corresponding N-domain sequence. The resultant chimeras C1-163Ndom-ACE, C417-579Ndom-ACE, and C583-623Ndom-ACE are processed to the cell surface of transfected Chinese hamster ovary cells, and are cleaved at the identical site as that of tACE. They show acquisition of N-domain-like catalytic properties. Homology modelling of the chimeric proteins reveals structural changes in regions required for tACE-specific catalytic activity. In contrast, C164-416Ndom-ACE and C191-214Ndom-ACE demonstrate defective intracellular processing and are neither enzymatically active nor shed. Therefore, critical elements within region D164V416 and more specifically I191T214 are required for the processing, cell-surface targeting, and enzyme activity of tACE, and cannot be substituted for by the homologous N-domain sequence | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C583-623Ndom-ACE | Homo sapiens | |
0.3 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, N-domain of tACE | Homo sapiens | |
0.4 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C1-163Ndom-ACE | Homo sapiens | |
0.5 | - |
hippuryl-His-Leu | 37°C, pH 8.3, N-domain of tACE | Homo sapiens | |
1.1 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C1-163Ndom-ACE | Homo sapiens | |
1.1 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C583623Ndom-ACE | Homo sapiens | |
1.1 | - |
hippuryl-His-Leu | 37°C, pH 8.3, tACE | Homo sapiens | |
1.2 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, ACE | Homo sapiens | |
2.7 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE | Homo sapiens | |
2.8 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C417579Ndom-ACE | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | both isoforms are shed from the cell surface via a sheddase-mediated cleavage, testis ACE is shed much more efficiently than somatic ACE | Homo sapiens | 9986 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P12821 | testis-specific isoform precursor | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Phe-His-Leu + H2O | - |
Homo sapiens | ? | - |
? | |
hippuryl-His-Leu + H2O | - |
Homo sapiens | hippuric acid + His-Leu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TACE | - |
Homo sapiens |
testis ACE | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, ACE | Homo sapiens | |
11.8 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE | Homo sapiens | |
12.9 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C583-623Ndom-ACE | Homo sapiens | |
15.1 | - |
hippuryl-His-Leu | 37°C, pH 8.3, chimeric enzyme C1163Ndom-ACE | Homo sapiens | |
24.5 | - |
hippuryl-His-Leu | 37°C, pH 8.3, tACE | Homo sapiens | |
34.4 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C417-579Ndom-ACE | Homo sapiens | |
36.8 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C583-623Ndom-ACE | Homo sapiens | |
73.2 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, ACE | Homo sapiens | |
97.5 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, chimeric enzyme C1-163Ndom-ACE | Homo sapiens | |
128 | - |
benzyloxycarbonyl-Phe-His-Leu | 37°C, pH 8.3, N-domain of tACE | Homo sapiens | |
262.5 | - |
hippuryl-His-Leu | 37°C, pH 8.3, N-domain of tACE | Homo sapiens |