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Literature summary for 3.4.15.1 extracted from
- Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resembling angiotensin I converting enzyme (2000), Comp. Biochem. Physiol. B, 126, 29-37.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Diazoacetylnorleucine methyl ester | 1.0 mM, activation to 115% of control | Alitta virens |  |
| diisopropylphosphoryl fluoride | 1.0 mM, activation to 116.3% of control | Alitta virens | |
| Soybean trypsin inhibitor | 0.5 mM, activation to 115% of control | Alitta virens | |
| tosylphenylalanine chloromethyl ketone | 1.0 mM, activation to 113% of control | Alitta virens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5.0 mM, complete inhibition | Alitta virens | |
| antipain | 0.1 mg/ml, 26% inhibition | Alitta virens | |
| captopril | 0.1 mM, complete inhibition | Alitta virens | |
| CuCl2 | 73% inhibition at 0.1 mM, 99% inhibition at 1.0 mM | Alitta virens | |
| EDTA | 1.0 mM, complete inhibition | Alitta virens | |
| leupeptin | 0.1 mg/ml, 18% inhibition | Alitta virens | |
| lisinopril | - |
Alitta virens | |
| additional information | enzyme is not affected by addition of 0.1-1.0 mM CaCl2, Mn2Cl2, MgCl2 or 0.1 mM ZnCl2 | Alitta virens | |
| PCMB | 1.0 mM, 42% inhibition | Alitta virens | |
| ZnCl2 | 1.0 mM, 98% inhibition | Alitta virens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
benzoyl-Gly-His-Leu | - |
Alitta virens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | 0.1-1.0 mM, 2fold activation | Alitta virens | |
| additional information | enzyme is not affected by addition of 0.1-1.0 mM CaCl2, Mn2Cl2, MgCl2 or 0.1 mM ZnCl2 | Alitta virens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Alitta virens |
| 71000 | - |
1 * 71000, SDS-PAGE | Alitta virens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin I + H2O | Alitta virens | the enzyme plays an important role in blood pressure homeostasis | angiotensin II + His-Leu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alitta virens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alitta virens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 466.8 | - |
- |
Alitta virens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin I + H2O | - |
Alitta virens | angiotensin II + His-Leu | - |
? | |
| angiotensin I + H2O | the enzyme plays an important role in blood pressure homeostasis | Alitta virens | angiotensin II + His-Leu | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O | cleavage sites: Arg-Pro-/-Lys-Pro-/-Gln-Gln-/-Phe-Phe-/-Gly-Leu-Met-NH2 | Alitta virens | ? | - |
? | |
| bradykinin + H2O | - |
Alitta virens | Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe | - |
? | |
| hippuryl-Gly-His-Leu + H2O | - |
Alitta virens | hippuric acid + His-Leu | - |
? | |
| hippuryl-His-Leu + H2O | - |
Alitta virens | hippuric acid + His-Leu | - |
? | |
| additional information | no cleavage of imido-bonds. No hydrolysis of angiotensin II and angiotensin III | Alitta virens | ? | - |
? | |
| Tyr-Gly-Gly-Phe-Leu + H2O | - |
Alitta virens | Tyr-Gly-Gly + Phe-Leu | - |
? | |
| Tyr-Gly-Gly-Phe-Met + H2O | - |
Alitta virens | Tyr-Gly-Gly + Phe-Met | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 71000, SDS-PAGE | Alitta virens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DCP | - |
Alitta virens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Alitta virens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 345 | - |
benzoyl-Gly-His-Leu | - |
Alitta virens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Alitta virens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000138 | - |
captopril | - |
Alitta virens | |
| 0.00000207 | - |
lisinopril | - |
Alitta virens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Alitta virens | - |
- |
4.5 |
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