Literature summary for 3.4.15.1 extracted from

Gordon, K.; Redelinghuys, P.; Schwager, S.L.; Ehlers, M.R.; Papageorgiou, A.C.; Natesh, R.; Acharya, K.R.; Sturrock, E.D.
Deglycosylation, processing and crystallization of human testis angiotensin-converting enzyme (2003), Biochem. J., 371, 437-442.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type enzyme and underglycosylated mutant enzymes in CHO cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour-diffusion hanging-drop method, wild-type and underglycosylated mutant enzymes. The truncated soluble enzyme DELTA1-36, expressed in the presence of the glucosidase-I inhibitor N-butyldeoxynojirimycin, retains the activity of the native enzyme and yields crystals belongining to the orthorhombic P2(1)2(1)2(1) space group. Cell dimensions: a = 56.47 A, b = 84.9 A, c = 144.99 A, alpha = 90°, beta = 90° and gamma = 90°	Homo sapiens

Crystallization (Comment)

Organism

vapour-diffusion hanging-drop method, wild-type and underglycosylated mutant enzymes. The truncated soluble enzyme DELTA1-36, expressed in the presence of the glucosidase-I inhibitor N-butyldeoxynojirimycin, retains the activity of the native enzyme and yields crystals belongining to the orthorhombic P2(1)2(1)2(1) space group. Cell dimensions: a = 56.47 A, b = 84.9 A, c = 144.99 A, alpha = 90°, beta = 90° and gamma = 90°

Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-values of underglycosylated mutant enzymes	Homo sapiens
2.7	-	hippuryl-His-Leu	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	integral membrane protein	Homo sapiens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	N-glycosylation of Asn72 or Asn109 are necessary and sufficient for the production of enzymatically active enzyme but glycosylation of Asn90 alone results in rapid intracellular degradation	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
testis	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuryl-His-Leu + H2O	-	Homo sapiens	hippuric acid + His-Leu	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	turnover numbers of underglycosylated mutant enzymes	Homo sapiens
196	-	hippuryl-His-Leu	-	Homo sapiens