Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression of enzyme ectodomain, comprising residues 31-766, in Pichia pastoris strain GS115, secretion to the culture medium | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant soluble enzyme ectodomain, vapour diffusion method, with 20-25% PEG 3350, 0.2 M MgCl2, Tris, pH 8.5, 15% glycerol, flash-frozen crystals for X-ray diffraction structure determination and analysis at 2.1 A resolution, also preparation and study of a mercury derivative | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ile-Pro-Ile | i.e. diprotin A, inhibition mechanism, binds covalently to Ser630 | Homo sapiens | |
NVP-DPP728 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0148 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, glycosylated recombinant soluble enzyme ectodomain | Homo sapiens | |
0.0155 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, deglycosylated recombinant soluble enzyme ectodomain bound to adenosine deaminase | Homo sapiens | |
0.0172 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, deglycosylated recombinant soluble enzyme ectodomain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | transmembrane protein | Homo sapiens | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27487 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | at Asn85, Asn150, and Asn229, glycosylation reduces the activity, and is not necessary for thermal stability | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme ectodomain from Pichia pastoris, to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | active site structure, containing the catalytic triad Ser630, Asp708, and His740, is located in a large cavity at the interface of the 2 domains, substrate binding and catalytic mechanism, serine exopeptidase | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro-7-amido-4-trifluoromethyl coumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-trifluoromethyl coumarin | - |
? | |
additional information | enzyme binds to adenosine deaminase, EC 3.5.4.4 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, structure and function of the 2 enzyme domains: the catayltic domain, with a alpha/beta hydrolase fold, and the propeller domain, with a 8fold repeat of a four-strand beta sheet motif | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
(GLP1)-degrading enzyme | - |
Homo sapiens |
dipeptidyl peptidase IV | - |
Homo sapiens |
DPP-IV | - |
Homo sapiens |
glucagon-like peptide 1-degrading enzyme | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
71 | - |
5 min, 50% remaining activity, structural features responsible for the thermal stability | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.3 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, glycosylated recombinant soluble enzyme ectodomain | Homo sapiens | |
39.6 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, deglycosylated recombinant soluble enzyme ectodomain bound to adenosine deaminase | Homo sapiens | |
43.1 | - |
Ala-Pro-7-amido-4-trifluoromethyl coumarin | pH 7.8, 25°C, deglycosylated recombinant soluble enzyme ectodomain | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000006 | - |
NVP-DPP728 | pH 7.8, 25°C, glycosylated recombinant soluble enzyme ectodomain | Homo sapiens | |
0.0046 | - |
Ile-Pro-Ile | pH 7.8, 25°C, glycosylated recombinant soluble enzyme ectodomain | Homo sapiens |