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Literature summary for 3.4.14.5 extracted from

  • Lorey, S.; Faust, J.; Mrestani-Klaus, C.; Kaehne, T.; Ansorge, S.; Neubert, K.; Buehling, F.
    Transcellular proteolysis demonstrated by novel cell surface-associated substrates of dipeptidyl peptidase IV (CD26) (2002), J. Biol. Chem., 277, 33170-33177.
    View publication on PubMed
Search for more literature for 3.4.14.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in CHO cells Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Nepsilon-nitrobenzyloxycarbonyl-Lys-thiazolide specific Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00058
-
 N-(Ala-Pro)-N'-6-(3,4-dichloromaleimidyl)-hexanoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0011
-
 N-(Gly-Pro)-N'-(4-chloromethyl)benzoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0013
-
 Gly-L-Pro-rhodamine 110-(N-pentyl)maleimide pH 7.2, 30°C Sus scrofa
0.0015
-
 N-(Gly-Pro)-N'-(5-bromopentanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0027
-
 N-(Gly-Pro)-N'-4-(maleimidyl)butanoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0038
-
 N-(Gly-Pro)-N'-(4-chlorobutanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0038
-
 N-(Gly-Pro)-N'-(5-chloropentanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0062
-
 N-(Gly-Pro)-N'-chloroacetyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.0174
-
 N-(Gly-Pro)-N'-acetyl-rhodamine 110 pH 7.2, 30°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cell surface-associated Sus scrofa 16020
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline enzyme is an ectopeptidase Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000025
-
 U937 cells, substrate Gly-Pro-4-nitroanilide Sus scrofa
51.9
-
 purified enzyme, substrate Gly-Pro-4-nitroanilide Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Pro-rhodamine 110-(N-pentyl)maleimide + H2O
-
 Sus scrofa Gly-L-Pro + rhodamine 110-(N-pentyl)maleimide
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Sus scrofa Gly-Pro + 4-nitroaniline
-
 ?
additional information larger compounds are cleaved with lower efficiency, importance of steric effects Sus scrofa ?
-
 ?
N-(Ala-Pro)-N'-6-(3,4-dichloromaleimidyl)-hexanoyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Ala-Pro + N-6-(3,4-dichloromaleimidyl)-hexanoyl-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-(4-chlorobutanoyl)-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-(4-chlorobutanoyl)-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-(4-chloromethyl)benzoyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-(4-chloromethyl)benzoyl-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-(5-bromopentanoyl)-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-(5-bromopentanoyl)-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-(5-chloropentanoyl)-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-(5-chloropentanoyl)-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-4-(maleimidyl)butanoyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-4-(maleimidyl)butanoyl-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-6-(maleimidyl)hexanoyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-6-(maleimidyl)hexanoyl-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-acetyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-acetyl-rhodamine 110
-
 ?
N-(Gly-Pro)-N'-chloroacetyl-rhodamine 110 + H2O fluorogenic substrate, membrane-anchored on the cell surface in a cell-based assay Sus scrofa Gly-Pro + N-chloroacetyl-rhodamine 110
-
 ?

Synonyms

Synonyms Comment Organism
CD26
-
 Sus scrofa
dipeptidyl peptidase IV
-
 Sus scrofa
DP IV
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37 assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 N-(Ala-Pro)-N'-6-(3,4-dichloromaleimidyl)-hexanoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
0.7
-
 N-(Gly-Pro)-N'-(4-chlorobutanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa
1.6
-
 N-(Gly-Pro)-N'-chloroacetyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
3.22
-
 N-(Gly-Pro)-N'-(5-bromopentanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa
3.4
-
 N-(Gly-Pro)-N'-(4-chloromethyl)benzoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
8
-
 N-(Gly-Pro)-N'-4-(maleimidyl)butanoyl-rhodamine 110 pH 7.2, 30°C Sus scrofa
8
-
 N-(Gly-Pro)-N'-(5-chloropentanoyl)-rhodamine 110 pH 7.2, 30°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Sus scrofa