Literature summary for 3.4.14.5 extracted from

Oefner, C.; D'Arcy, A.; Mac Sweeney, A.; Pierau, S.; Gardiner, R.; Dale, G.E.
High-resolution structure of human apo dipeptidyl peptidase IV/CD26 and its complex with 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano-(S)-pyrrol idine (2003), Acta Crystallogr. Sect. D, 59, 1206-1212.

Cloned(Commentary)

Cloned (Comment)	Organism
expression as soluble extracellular enzyme domain	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
20 mg/ml purified soluble extracellular domain apoprotein, glycosidase endo-F treated, in 25 mM Tris, pH 7.5, 100 mM NaCl, 2 mM Tris(2-carboxyethyl)phosphine hydrochloride, i.e. TCEP, 5% glycerol, modified microbatch method, hanging drop vapour diffusion method, from 25% PEG 3350, 200 mM MgCl2, 100 mM HEPES, pH 7.5, complexing with inhibitor 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano(S)-pyrrolidine, several days, X-ray diffraction structure determination and analysis at 1.9 A resolution, in cryoprotectant solution containing 25% PEG 3350, 20% 1,6-hexanediol, at 100 K	Homo sapiens

Crystallization (Comment)

Organism

20 mg/ml purified soluble extracellular domain apoprotein, glycosidase endo-F treated, in 25 mM Tris, pH 7.5, 100 mM NaCl, 2 mM Tris(2-carboxyethyl)phosphine hydrochloride, i.e. TCEP, 5% glycerol, modified microbatch method, hanging drop vapour diffusion method, from 25% PEG 3350, 200 mM MgCl2, 100 mM HEPES, pH 7.5, complexing with inhibitor 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano(S)-pyrrolidine, several days, X-ray diffraction structure determination and analysis at 1.9 A resolution, in cryoprotectant solution containing 25% PEG 3350, 20% 1,6-hexanediol, at 100 K

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano(S)-pyrrolidine	competitive	Homo sapiens
2-cyanopyrrolidines	-	Homo sapiens
alpha-amino boronic acid analogues of pyrrolidides	-	Homo sapiens
alpha-amino boronic acid analogues of thiazolidides	-	Homo sapiens
diaryl phosphonates	-	Homo sapiens
Diprotin A	-	Homo sapiens
Diprotin B	-	Homo sapiens
pyrrolidine-carbonitrile derivatives	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	soluble form from seminal plasma and serum	Homo sapiens	-	-
membrane	type II transmembrane protein	Homo sapiens	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	-	recombinant extracellular domain, gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble extracellular domain from cell culture supernatant	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline	serine protease, catalytic triad Ser630, Asp708, His740, active site architecture	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
epithelium	-	Homo sapiens	-
exocrine acinar cell	-	Homo sapiens	-
kidney	-	Homo sapiens	-
lymphocyte	-	Homo sapiens	-
seminal plasma	-	Homo sapiens	-
serum	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
incretin hormone glucagon-like peptide 1 + H2O	i.e. GLP-1, degradation, regulation of activity	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CD26	-	Homo sapiens
dipeptidyl peptidase IV	-	Homo sapiens
DPP-IV	-	Homo sapiens