General Stability | Organism |
---|---|
treatment with chymotrypsin, trypsin, protease from Streptomyces griseus and papain reduce 18-22% of the activity after 22 h after incubation | Streptococcus salivarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM CuSO4, 15% inhibition | Streptococcus salivarius | |
diisopropylfluoride | - |
Streptococcus salivarius | |
L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone | - |
Streptococcus salivarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, SDS-PAGE | Streptococcus salivarius |
109000 | - |
gel filtration | Streptococcus salivarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus salivarius | - |
HHT | - |
Streptococcus salivarius HHT | - |
HHT | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus salivarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.28 | - |
- |
Streptococcus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro-4-nitroanilide + H2O | 48.6% of the activity with Gly-Pro-4-nitroanilide | Streptococcus salivarius | Arg-Pro + 4-nitroaniline | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 48.6% of the activity with Gly-Pro-4-nitroanilide | Streptococcus salivarius HHT | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus salivarius | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus salivarius HHT | Gly-Pro + 4-nitroaniline | - |
? | |
Lys-Pro-4-nitroanilide + H2O | 48.6% of the activity with Gly-Pro-4-nitroanilide | Streptococcus salivarius | Lys-Pro + 4-nitroaniline | - |
? | |
Lys-Pro-4-nitroanilide + H2O | 48.6% of the activity with Gly-Pro-4-nitroanilide | Streptococcus salivarius HHT | Lys-Pro + 4-nitroaniline | - |
? | |
additional information | specifically hydrolyzes N-terminal X-Pro from X-Pro-4-nitroanilides | Streptococcus salivarius | ? | - |
? | |
additional information | specifically hydrolyzes N-terminal X-Pro from X-Pro-4-nitroanilides | Streptococcus salivarius HHT | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-PAGE | Streptococcus salivarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Streptococcus salivarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 70% of maximal activity, 50°C: about 75% of maximal activity | Streptococcus salivarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
relatively stable below | Streptococcus salivarius |
55 | - |
15 min, about 95% loss of activity | Streptococcus salivarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Streptococcus salivarius |