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Literature summary for 3.4.14.5 extracted from
- Purification and properties of dipeptidyl peptidase IV from Streptococcus mitis ATCC 9811 (1981), Arch. Biochem. Biophys., 210, 230-237.
General Stability
| General Stability | Organism |
|---|---|
| treatment with trypsin, chymotrypsin A, pronase-P and papain completely destroy the activity | Streptococcus mitis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Streptococcus mitis |  |
| Cu2+ | - |
Streptococcus mitis | |
| diisopropyl fluorophosphate | - |
Streptococcus mitis | |
| EDTA | - |
Streptococcus mitis | |
| Hg2+ | - |
Streptococcus mitis | |
| Pb2+ | - |
Streptococcus mitis | |
| Zn2+ | - |
Streptococcus mitis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
Gly-Pro-4-nitroanilide | - |
Streptococcus mitis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | slight increase in activity | Streptococcus mitis | |
| Mn2+ | slight increase in activity | Streptococcus mitis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Streptococcus mitis |
| 120000 | - |
gel filtration | Streptococcus mitis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mitis | - |
ATCC 9811 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | no carbohydrate | Streptococcus mitis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus mitis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56.2 | - |
- |
Streptococcus mitis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-4-nitroanilide + H2O | 20% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Ala-Ala + 4-nitroaniline | - |
? | |
| Glu-Pro-4-nitroanilide + H2O | 23% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Glu-Pro + 4-nitroaniline | - |
? | |
| Gly-hydroxyproline-4-nitroanilide + H2O | 10% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Gly-hydroxyproline + 4-nitroaniline | - |
? | |
| Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus mitis | Gly-Pro + 4-nitroaniline | - |
? | |
| Gly-Pro-Leu-Gly-Pro + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | ? | - |
? | |
| Lys-Pro-4-nitroanilide + H2O | 65% of the activity with Gly-Pro-4-nitroanilide | Streptococcus mitis | Lys-Pro + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Streptococcus mitis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Streptococcus mitis |
| 55 | - |
10 min, activity is almost lost | Streptococcus mitis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.7 | - |
Streptococcus mitis |
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