Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
D22H | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
D320A | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
D320N | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
H150A | mutant with more than doubled Km-values | Streptomyces coelicolor |
H150N | mutant with more than doubled Km-values | Streptomyces coelicolor |
R223K | mutant with about 3fold reduced activity compared to wild-type enzyme | Streptomyces coelicolor |
R223M | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | - |
Streptomyces coelicolor | |
phosphinate mimic of L-Ala-D-Asp | - |
Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-Arg-D-Asp | - |
Streptomyces coelicolor | |
0.17 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
0.37 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
0.55 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
0.56 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
0.75 | - |
L-Leu-D-Ala | - |
Streptomyces coelicolor | |
0.89 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
1.4 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
1.8 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
2 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
2 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
2.3 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
2.4 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
4.3 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
4.4 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
4.9 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
8.8 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
9.5 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
13.8 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
16 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, size exclusion chromatography, ion exchange chromatography and gel filtration | Streptomyces coelicolor |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | D-Ala + D-Ala | - |
? | |
D-Ala-L-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | D-Ala + L-Ala | - |
? | |
Gly-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | Gly + D-Leu | - |
? | |
L-Ala-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + D-Ala | - |
? | |
L-Ala-L-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Ala | - |
? | |
L-Ala-L-Arg + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Arg | - |
? | |
L-Ala-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Asp | - |
? | |
L-Ala-L-Gln + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Gln | - |
? | |
L-Ala-L-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Glu | - |
? | |
L-Ala-L-His + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-His | - |
? | |
L-Ala-L-Lys + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Lys | - |
? | |
L-Arg-D-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + D-Asp | - |
? | |
L-Arg-Gly + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + Gly | - |
? | |
L-Arg-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + L-Asp | - |
? | |
L-Asn-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Asn + D-Glu | - |
? | |
L-Leu-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Ala | - |
? | |
L-Leu-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Glu | - |
? | |
L-Leu-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Leu | - |
? | |
L-Leu-D-Ser + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Ser | - |
? | |
L-Leu-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + L-Asp | - |
? | |
L-Leu-L-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + L-Leu | - |
? | |
L-Met-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + D-Glu | - |
? | |
L-Met-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + D-Leu | - |
? | |
L-Met-L-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + L-Leu | - |
? | |
L-Tyr-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Tyr + D-Leu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
renal dipeptidase | - |
Streptomyces coelicolor |
Sco3058 | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-Arg-L-Asp | mutant D22H, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
additional information | - |
L-Arg-L-Asp | mutant D320A, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
additional information | - |
L-Arg-L-Asp | mutant D320N, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
additional information | - |
L-Arg-L-Asp | mutant R223M, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
0.4 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
0.7 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
7.2 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
21 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
24 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
107 | - |
L-Arg-D-Asp | - |
Streptomyces coelicolor | |
132 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
135 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
140 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
140 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor | |
160 | - |
L-Leu-D-Ala | - |
Streptomyces coelicolor | |
160 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
194 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
220 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
288 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
310 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
340 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
400 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
750 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
1400 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
metabolism | glutathione metabolism | Streptomyces coelicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
D-Ala-L-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
0.046 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
0.072 | - |
L-Ala-L-Lys | pH 7.5, 30°C | Streptomyces coelicolor | |
0.13 | - |
D-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
0.14 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
0.18 | - |
L-Ala-L-Arg | pH 7.5, 30°C | Streptomyces coelicolor | |
0.45 | - |
L-Ala-L-His | pH 7.5, 30°C | Streptomyces coelicolor | |
1.7 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
8.4 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor | |
14 | - |
L-Ala-L-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
22 | - |
L-Arg-Gly | pH 7.5, 30°C | Streptomyces coelicolor | |
24 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
33 | - |
Gly-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
56 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
72 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
83 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
100 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
100 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
120 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
140 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
150 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
170 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
200 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
210 | - |
L-Leu-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
250 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
350 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
380 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
760 | - |
L-Arg-D-Asp | pH 7.5, 30°C | Streptomyces coelicolor |