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Literature summary for 3.4.13.19 extracted from

  • Cummings, J.A.; Nguyen, T.T.; Fedorov, A.A.; Kolb, P.; Xu, C.; Fedorov, E.V.; Shoichet, B.K.; Barondeau, D.P.; Almo, S.C.; Raushel, F.M.
    Structure, mechanism, and substrate profile for Sco3058: the closest bacterial homologue to human renal dipeptidase (2010), Biochemistry, 49, 611-622.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
D22H mutant with no residual enzyme acitvity Streptomyces coelicolor
D320A mutant with no residual enzyme acitvity Streptomyces coelicolor
D320N mutant with no residual enzyme acitvity Streptomyces coelicolor
H150A mutant with more than doubled Km-values Streptomyces coelicolor
H150N mutant with more than doubled Km-values Streptomyces coelicolor
R223K mutant with about 3fold reduced activity compared to wild-type enzyme Streptomyces coelicolor
R223M mutant with no residual enzyme acitvity Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Streptomyces coelicolor
phosphinate mimic of L-Ala-D-Asp
-
 Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-Arg-D-Asp
-
 Streptomyces coelicolor
0.17
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
0.37
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
0.55
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
0.56
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
0.75
-
 L-Leu-D-Ala
-
 Streptomyces coelicolor
0.89
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
1.4
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
1.8
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
2
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
2
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
2.3
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
2.4
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
4.3
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
4.4
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
4.9
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
8.8
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
9.5
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
13.8
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor
16
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, size exclusion chromatography, ion exchange chromatography and gel filtration Streptomyces coelicolor

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor D-Ala + D-Ala
-
 ?
D-Ala-L-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor D-Ala + L-Ala
-
 ?
Gly-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor Gly + D-Leu
-
 ?
L-Ala-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + D-Ala
-
 ?
L-Ala-L-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Ala
-
 ?
L-Ala-L-Arg + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Arg
-
 ?
L-Ala-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Asp
-
 ?
L-Ala-L-Gln + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Gln
-
 ?
L-Ala-L-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Glu
-
 ?
L-Ala-L-His + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-His
-
 ?
L-Ala-L-Lys + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Ala + L-Lys
-
 ?
L-Arg-D-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + D-Asp
-
 ?
L-Arg-Gly + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + Gly
-
 ?
L-Arg-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Arg + L-Asp
-
 ?
L-Asn-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Asn + D-Glu
-
 ?
L-Leu-D-Ala + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Ala
-
 ?
L-Leu-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Glu
-
 ?
L-Leu-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Leu
-
 ?
L-Leu-D-Ser + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + D-Ser
-
 ?
L-Leu-L-Asp + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + L-Asp
-
 ?
L-Leu-L-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Leu + L-Leu
-
 ?
L-Met-D-Glu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + D-Glu
-
 ?
L-Met-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + D-Leu
-
 ?
L-Met-L-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Met + L-Leu
-
 ?
L-Tyr-D-Leu + H2O assay at 30°C, pH 7.5 Streptomyces coelicolor L-Tyr + D-Leu
-
 ?

Synonyms

Synonyms Comment Organism
renal dipeptidase
-
 Streptomyces coelicolor
Sco3058
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 L-Arg-L-Asp mutant D22H, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
additional information
-
 L-Arg-L-Asp mutant D320A, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
additional information
-
 L-Arg-L-Asp mutant D320N, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
additional information
-
 L-Arg-L-Asp mutant R223M, value below 0.03, pH 7.5, 30°C Streptomyces coelicolor
0.4
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
0.7
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
7.2
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
21
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
24
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
107
-
 L-Arg-D-Asp
-
 Streptomyces coelicolor
132
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
135
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
140
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
140
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor
160
-
 L-Leu-D-Ala
-
 Streptomyces coelicolor
160
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
194
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
220
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
288
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
310
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
340
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
400
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
750
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
1400
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptomyces coelicolor

General Information

General Information Comment Organism
metabolism glutathione metabolism Streptomyces coelicolor

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0022
-
 D-Ala-L-Ala pH 7.5, 30°C Streptomyces coelicolor
0.046
-
 L-Arg-L-Asp mutant R223K, pH 7.5, 30°C Streptomyces coelicolor
0.072
-
 L-Ala-L-Lys pH 7.5, 30°C Streptomyces coelicolor
0.13
-
 D-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
0.14
-
 L-Arg-L-Asp mutant H150N, pH 7.5, 30°C Streptomyces coelicolor
0.18
-
 L-Ala-L-Arg pH 7.5, 30°C Streptomyces coelicolor
0.45
-
 L-Ala-L-His pH 7.5, 30°C Streptomyces coelicolor
1.7
-
 L-Arg-L-Asp mutant H150A, pH 7.5, 30°C Streptomyces coelicolor
8.4
-
 L-Ala-L-Gln pH 7.5, 30°C Streptomyces coelicolor
14
-
 L-Ala-L-Ala pH 7.5, 30°C Streptomyces coelicolor
22
-
 L-Arg-Gly pH 7.5, 30°C Streptomyces coelicolor
24
-
 L-Ala-L-Glu pH 7.5, 30°C Streptomyces coelicolor
33
-
 Gly-D-Leu pH 7.5, 30°C Streptomyces coelicolor
56
-
 L-Leu-D-Leu pH 7.5, 30°C Streptomyces coelicolor
72
-
 L-Arg-L-Asp wild-type enzyme, pH 7.5, 30°C Streptomyces coelicolor
83
-
 L-Leu-L-Asp pH 7.5, 30°C Streptomyces coelicolor
100
-
 L-Leu-L-Leu pH 7.5, 30°C Streptomyces coelicolor
100
-
 L-Ala-L-Asp pH 7.5, 30°C Streptomyces coelicolor
120
-
 L-Asn-D-Glu pH 7.5, 30°C Streptomyces coelicolor
140
-
 L-Met-L-Leu pH 7.5, 30°C Streptomyces coelicolor
150
-
 L-Tyr-D-Leu pH 7.5, 30°C Streptomyces coelicolor
170
-
 L-Ala-D-Ala pH 7.5, 30°C Streptomyces coelicolor
200
-
 L-Leu-D-Ser pH 7.5, 30°C Streptomyces coelicolor
210
-
 L-Leu-D-Ala pH 7.5, 30°C Streptomyces coelicolor
250
-
 L-Leu-D-Glu pH 7.5, 30°C Streptomyces coelicolor
350
-
 L-Met-D-Leu pH 7.5, 30°C Streptomyces coelicolor
380
-
 L-Met-D-Glu pH 7.5, 30°C Streptomyces coelicolor
760
-
 L-Arg-D-Asp pH 7.5, 30°C Streptomyces coelicolor