Literature summary for 3.4.13.19 extracted from

Dolenc, I.; Pain, R.; Turk, V.
Presence of the propeptide on recombinant lysosomal dipeptidase controls both activation and dimerization (2007), Biol. Chem., 388, 47-51.

Search for more literature for 3.4.13.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Codon Plus-RIL cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	includes a bis-zinc-containing active center, in which both zinc ions have catalytic properties, folding efficiency is not improved by including up to 25 mM ZnCl2 in the folding buffer, but concentrations above 50 mM ZnCl2 cause precipitation of the protein	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ultracentrifugation and Superose 12 (10/300) gel filtration	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
in 20 mM Tris-HCl, pH 8.0, 500 mM arginine, containing 1 mM reduced glutathione, 0.2 mM oxidized glutathione, and sodium chloride	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hemoglobin + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
LDP	-	Homo sapiens
lysosomal dipeptidase	-	Homo sapiens
Ser-Met dipeptidase	original name	Homo sapiens

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Release 2023.1 (January 2023)