General Stability | Organism |
---|---|
substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Geobacillus stearothermophilus | |
Ca2+ | - |
Geobacillus stearothermophilus | |
Cu2+ | - |
Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
Mg2+ | - |
Geobacillus stearothermophilus | |
Zn2+ | slight | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates | Geobacillus stearothermophilus | |
Co2+ | activates,substitution of Co2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein | Geobacillus stearothermophilus | |
Mn2+ | activates | Geobacillus stearothermophilus | |
Mn2+ | the Mn-reconstituted enzyme has nearly twice the activity of the original Zn-enzyme, substitution of Mn2+ for Zn2+ considerably loweres the thermostability of the enzyme without affecting the overall conformation of the protein | Geobacillus stearothermophilus | |
Zinc | the metalloenzyme contains 1 gatom of zinc per mol of subunit, the prosthetic Zn plays dual roles in conformational stability and catalysis of the thermostable dipeptidase | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein | Geobacillus stearothermophilus |
70 | - |
30 min, about 15% loss of activity of the native enzyme, about 55% of the activity of the Co2+-reconstituted enzyme, about 75% loss of activity of the Mn2+-reconstituted enzyme | Geobacillus stearothermophilus |