General Stability | Organism |
---|---|
extended dialysis against nonchelating buffers does not result in loss of activity | Sus scrofa |
no loss of activity upon lyophilization | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Sus scrofa | |
acetate | - |
Sus scrofa | |
Br- | - |
Sus scrofa | |
Cd2+ | - |
Sus scrofa | |
Cl- | - |
Sus scrofa | |
CN- | - |
Sus scrofa | |
F- | - |
Sus scrofa | |
HCO3- | - |
Sus scrofa | |
I- | - |
Sus scrofa | |
additional information | inhibition by monovalent cations in decreasing order CN-, SCN-, N3-, I-, NO3-, HCO3-, Br-, OAc-, Cl-, F- | Sus scrofa | |
N3- | - |
Sus scrofa | |
Ni2+ | - |
Sus scrofa | |
NO3- | - |
Sus scrofa | |
nucleotides | - |
Sus scrofa | |
phosphate | - |
Sus scrofa | |
SCN- | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
glycyldehydrophenylalanine | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Sus scrofa | |
Mn2+ | activates | Sus scrofa | |
Zinc | contains 1 mol of zinc per mol of enzyme | Sus scrofa | |
Zinc | no absolute specificity for Zn2+ activation | Sus scrofa | |
Zinc | contains 1 mol of zinc per mol of enzyme of MW 47200 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47200 | - |
approach to equilibrium measurement | Sus scrofa |
87000 | 93000 | sedimentation equilibrium measurement, gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
frozen, in dilute solution, 0.13 mg/ml Tris-HCl buffer, pH 8, loses two-third of its activity after 6 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-D-Phe + H2O | - |
Sus scrofa | Gly + D-Phe | - |
? | |
Gly-Gly + H2O | - |
Sus scrofa | Gly + Gly | - |
? | |
glycyldehydrophenylalanine + H2O | - |
Sus scrofa | ? | - |
? | |
L-Ala-Gly + H2O | - |
Sus scrofa | L-Ala + Gly | - |
? | |
additional information | broad specificity | Sus scrofa | ? | - |
? | |
additional information | no esterase activity | Sus scrofa | ? | - |
? | |
additional information | no hydrolysis of proteins | Sus scrofa | ? | - |
? | |
additional information | no hydrolysis of tripeptides | Sus scrofa | ? | - |
? | |
additional information | no hydrolysis of leucinamide | Sus scrofa | ? | - |
? | |
additional information | no reaction with leucinamide | Sus scrofa | ? | - |
? | |
additional information | requires the N-terminal amino acid of the dipeptide in the L-configuration | Sus scrofa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
glycyldehydrophenylalanine | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
inactivation below | Sus scrofa |
8 | - |
stable at | Sus scrofa |