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Literature summary for 3.3.2.6 extracted from

  • Stsiapanava, A.; Tholander, F.; Kumar, R.B.; Qureshi, A.A.; Niegowski, D.; Hasan, M.; Thunnissen, M.; Haeggstroem, J.Z.; Rinaldo-Matthis, A.
    Product formation controlled by substrate dynamics in leukotriene A4 hydrolase (2014), Biochim. Biophys. Acta, 1844, 439-446.
    View publication on PubMed
Search for more literature for 3.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM101 cells Xenopus laevis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM Bis-Tris pH 6.5, 20%(w/v) PEG 3350 Xenopus laevis

Protein Variants

Protein Variants Comment Organism
F375Y the mutation abolishes the formation of 5(S),12R-dihydroxy-6,10-trans-8,14-ciseicosatetraenoic acid Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
 L-alanine-4-nitroanilide mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis
0.0026
-
 L-alanine-4-nitroanilide wild type enzyme, at pH 7.8 and 22°C Xenopus laevis
0.004
-
 leukotriene A4 mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis
0.045
-
 leukotriene A4 wild type enzyme, at pH 7.8 and 22°C Xenopus laevis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Xenopus laevis
-
 leukotriene B4 + 5(S),12R-dihydroxy-6,10-trans-8,14-ciseicosatetraenoic acid product ratio 9:1 ?

Organism

Organism UniProt Comment Textmining
Xenopus laevis Q6IP81
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, and MonoQ column chromatography Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-nitroanilide + H2O
-
 Xenopus laevis L-alanine + 4-nitroaniline
-
 ?
leukotriene A4 + H2O
-
 Xenopus laevis leukotriene B4 + 5(S),12R-dihydroxy-6,10-trans-8,14-ciseicosatetraenoic acid product ratio 9:1 ?

Synonyms

Synonyms Comment Organism
leukotriene A4 hydrolase a bifunctional zinc metalloenzyme with both an epoxide hydrolase and an aminopeptidase activity Xenopus laevis
LTA4H
-
 Xenopus laevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 leukotriene A4 mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis
3.44
-
 L-alanine-4-nitroanilide mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis
4.3
-
 L-alanine-4-nitroanilide wild type enzyme, at pH 7.8 and 22°C Xenopus laevis
20.1
-
 leukotriene A4 wild type enzyme, at pH 7.8 and 22°C Xenopus laevis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
 leukotriene A4 wild type enzyme, at pH 7.8 and 22°C Xenopus laevis
0.8
-
 leukotriene A4 mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis
1.7
-
 L-alanine-4-nitroanilide wild type enzyme, at pH 7.8 and 22°C Xenopus laevis
3.96
-
 L-alanine-4-nitroanilide mutant enzyme F375Y, at pH 7.8 and 22°C Xenopus laevis