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Literature summary for 3.3.2.6 extracted from

  • Thompson, M.W.; Archer, E.D.; Romer, C.E.; Seipelt, R.L.
    A conserved tyrosine residue of Saccharomyces cerevisiae leukotriene A4 hydrolase stabilizes the transition state of the peptidase activity (2006), Peptides, 27, 1701-1709.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pYES2.1-Topo vector for expression of the recombinant proteins in Saccharomyces cerevisiae 1971 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y244F leukotriene A4 hydrolase mutant Saccharomyces cerevisiae
Y256F leukotriene A4 hydrolase mutant Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
Saccharomyces cerevisiae
puromycin
-
Saccharomyces cerevisiae
RB-3014
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O Saccharomyces cerevisiae substrate for epoxide hydrolase activity (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using metal affinity chromatography, the His tag is removed by digestion with His6-TEV protease Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O substrate for epoxide hydrolase activity Saccharomyces cerevisiae (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
leukotriene-A4 hydrolase
-
Saccharomyces cerevisiae
LTA4H
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
activity assay Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000087
-
RB-3014 wild-type LTA4H Saccharomyces cerevisiae
0.00074
-
RB-3014 mutant Y456F Saccharomyces cerevisiae
0.0027
-
bestatin wild-type LTA4H Saccharomyces cerevisiae
0.0037
-
RB-3014 mutant Y244F Saccharomyces cerevisiae
0.049
-
bestatin mutant Y456F Saccharomyces cerevisiae
0.0765
-
bestatin mutant Y244F Saccharomyces cerevisiae
2.37
-
puromycin mutant Y244F Saccharomyces cerevisiae
3.16
-
puromycin wild-type LTA4H Saccharomyces cerevisiae
3.24
-
puromycin mutant Y456F Saccharomyces cerevisiae