Literature summary for 3.3.2.6 extracted from

Rudberg, P.C.; Tholander, F.; Thunnissen, M.M.; Haeggstrom, J.Z.
Leukotriene A4 hydrolase/aminopeptidase. Glutamate 271 is a catalytic residue with specific roles in two distinct enzyme mechanisms (2002), J. Biol. Chem., 277, 1398-1404.

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Application

Application	Comment	Organism
medicine	the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type enzyme and mutant E271Q	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E271A	complete loss of the epoxide hydrolase and aminopeptidase activities	Homo sapiens
E271D	complete loss of the epoxide hydrolase and aminopeptidase activities	Homo sapiens
E271Q	complete loss of the epoxide hydrolase and aminopeptidase activities	Homo sapiens
G268A	epoxide hydrolase activity: 97% of Km of wild type enzyme, aminopeptidase activity: 73% of Km of wild type enzyme	Homo sapiens
G269A	epoxide hydrolase activity: 202% of Km of wild type enzyme, aminopeptidase activity: 66% of Km of wild type enzyme	Homo sapiens
M270L	epoxide hydrolase activity: 21% of Km of wild type enzyme, aminopeptidase activity: 189% of Km of wild type enzyme	Homo sapiens
N272A	epoxide hydrolase activity: 30% of Km of wild type enzyme, aminopeptidase activity: 221% of Km of wild type enzyme	Homo sapiens
Q136A	epoxide hydrolase activity: 67% of Km of wild type enzyme, aminopeptidase activity: 108% of Km of wild type enzyme	Homo sapiens
Q136L	epoxide hydrolase activity: 179% of Km of wild type enzyme	Homo sapiens
Q136N	epoxide hydrolase activity: 18% of Km of wild type enzyme, aminopeptidase activity: 24% of Km of wild type enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	0.028	leukotriene A4	pH 8.0, wild-type enzyme in comparison to mutant enzymes	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
leukotriene A4 + H2O	Homo sapiens	-	leukotriene B4	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
leukotriene A4 + H2O = leukotriene B4	mechanistic models, Glu271 is specifically required for the epoxid hydrolase activity and peptidase activity	Homo sapiens	a
leukotriene A4 + H2O = leukotriene B4	bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
leukotriene A4 + H2O	-	Homo sapiens	leukotriene B4	-	?
additional information	-	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.21	0.47	leukotriene A4	pH 8.0, wild-type enzyme in comparison to mutant enzymes	Homo sapiens

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Release 2023.1 (January 2023)