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Literature summary for 3.3.2.6 extracted from

  • Wetterholm, A.; Medina, J.F.; Radmark, O.; Shapiro, R.; Haeggström, J.Z.; Vallee, B.L.; Samuelsson, B.
    Recombinant mouse leukotriene A4 hydrolase: a zinc metalloenzyme with dual enzymatic activities (1991), Biochim. Biophys. Acta, 1080, 96-102.
    View publication on PubMed
Search for more literature for 3.3.2.6

Application

Application Comment Organism
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus
0.005
-
 leukotriene A4 pH 8.0, 0°C Mus musculus
0.68
-
 L-alanine-4-nitroanilide pH 8.0, 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt Mus musculus
Zinc apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt Mus musculus
Zinc zinc content: 1 mol of zinc per mol of enzyme Mus musculus
Zinc zinc protein Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 x * 69000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus Mus musculus

Reaction

Reaction Comment Organism Reaction ID
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Mus musculus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.185
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-nitroanilide + H2O
-
 Mus musculus L-alanine + 4-nitroaniline
-
 ?
leukotriene A4 + H2O
-
 Mus musculus leukotriene B4
-
 ?
additional information
-
 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 69000, SDS-PAGE Mus musculus

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus chromatofocusing
-
 5.5