Literature summary for 3.2.2.27 extracted from

Wang, L.; Zhang, H.; Xie, Y.; Chen, H.; Ren, C.; Chen, X.
Target-mediated hyperbranched amplification for sensitive detection of human alkyladenine DNA glycosylase from HeLa cells (2019), Talanta, 194, 846-851 .

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Application

Application	Comment	Organism
analysis	fluorescence method for sensitive detection of uracil-DNA glycosylase using TdT-activated Endonuclease IV-assisted hyperbranched signal amplification. The method exhibits high sensitivity with a limit of detection of 0.090 U/ml for pure uracil-DNA glycosylase and shows a dynamic range from 0.1 to 50 U/ml, and can be applied for accurate detection of uracil-DNA glycosylase in HeLa nuclear extract. The method can be used for discrimination of uracil-DNA glycosylase from other DNA glycosylases	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P13051	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

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Release 2023.1 (January 2023)