Cloned (Comment) | Organism |
---|---|
NEH1 is cloned, sequenced and expressed in Escherichia coli BL21(DE3), chromosomal location: 15q25 | Homo sapiens |
NEH2, chromosomal location: 4q35 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | NEH1 is not localized in mitochondria | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no zinc finger | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36800 | - |
x * 36800, NEH2, calculated from the amino acid sequence | Homo sapiens |
43582 | - |
x * 43582, recombinant NEH1, calculated from the amino acid sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Homo sapiens | involved in replication-associated repair of oxidized bases | ? | - |
? | |
DNA + H2O | Homo sapiens | DNA base excision repair enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q969S2 | human nei homolog NEH2 | - |
Homo sapiens | Q96FI4 | human nei homolog NEH1 | - |
Purification (Comment) | Organism |
---|---|
recombinant NEH1, expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | moderate expression of NEH1 | Homo sapiens | - |
leukocyte | low expression of NEH1 | Homo sapiens | - |
liver | highest expression of NEH1 in liver, pancreas and thymus | Homo sapiens | - |
MRC-5 cell | primary human diploid fibroblasts, S phase-specific expression of NEH1 | Homo sapiens | - |
ovary | moderate expression of NEH1 | Homo sapiens | - |
pancreas | highest expression of NEH1 in liver, pancreas and thymus | Homo sapiens | - |
prostate | moderate expression of NEH1 | Homo sapiens | - |
spleen | moderate expression of NEH1 | Homo sapiens | - |
testis | low expression of NEH1 | Homo sapiens | - |
thymus | highest expression of NEH1 in liver, pancreas and thymus | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | substrate specificity | Homo sapiens | ? | - |
? | |
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Homo sapiens | ? | - |
? | |
DNA + H2O | excises formamidopyrimidines from damaged DNA and oxidized pyrimidines and 8-oxoguanine from oligodeoxynucleotides, role of the N-terminal Pro as its active site | Homo sapiens | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Homo sapiens | ? | - |
? | |
DNA + H2O | involved in replication-associated repair of oxidized bases | Homo sapiens | ? | - |
? | |
DNA + H2O | DNA base excision repair enzyme | Homo sapiens | ? | - |
? | |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O | FapyGua | Homo sapiens | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O | FapyAde | Homo sapiens | 4,6-diamino-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 5-hydroxy-2'-deoxyuridine + H2O | dublex oligodeoxynucleotide containing 5-hydroxyuracil when paired with G | Homo sapiens | ? | - |
? | |
DNA containing 7-hydro-8-oxoguanine residues + H2O | excises 8-oxoguanine from 31mer oligodeoxynucleotide, most active with G, followed by T, opposite the lesion, weak activity with C or A opposite | Homo sapiens | DNA + 7-hydro-8-oxoguanine | - |
? | |
DNA containing dihydrouracil residues + H2O | active as a DNA glycosylase/AP lyase with dihydrouracil 31mer dublex oligo substrate, similar activity with A, C or G opposite the lesion, reduced with T opposite | Homo sapiens | ? | - |
? | |
additional information | not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36800, NEH2, calculated from the amino acid sequence | Homo sapiens |
? | x * 43582, recombinant NEH1, calculated from the amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NEH1 | human homolog to MutM and Nei | Homo sapiens |
NEH2 | human homolog to MutM and Nei | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |