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Literature summary for 3.2.2.23 extracted from

  • Rabow, L.; Venkataraman, R.; Kow, Y.W.
    Mechanism of action of Escherichia coli formamidopyrimidine N-glycosylase: Role of K155 in substrate binding and product release (2001), Prog. Nucleic Acid Res. Mol. Biol., 68, 223-234.
    View publication on PubMed
Search for more literature for 3.2.2.23

Protein Variants

Protein Variants Comment Organism
K155A mutant enzyme with decreased N-glycosylase and increased AP lyase activity, it dissociates prematurely from the covalent enzyme-DNA complex leading to a higher turnover number for DNA containing an AP site Escherichia coli
K57A mutant with about 15% of wild-type activity in both N-glycosylase and AP lyase activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for DNA substrate is affected by the base opposite the lesion Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions Escherichia coli ?
-
 ?
DNA + H2O role of Lys-155 for substrate binding and product release, AP lyase mechanism Escherichia coli ?
-
 ?
DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O FapyGua Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
 ?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O FapyAde Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
 ?
DNA containing 7-hydro-8-oxoguanine residues + H2O
-
 Escherichia coli DNA + 7-hydro-8-oxoguanine
-
 ?
DNA containing ring-opened N7-methylguanine residues + H2O
-
 Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli