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Literature summary for 3.2.1.8 extracted from

  • Kuramochi, K.; Uchimura, K.; Kurata, A.; Kobayashi, T.; Hirose, Y.; Miura, T.; Kishimoto, N.; Usami, R.; Horikoshi, K.
    A high-molecular-weight, alkaline, and thermostable beta-1,4-xylanase of a subseafloor Microcella alkaliphila (2016), Extremophiles, 20, 471-478.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzyl bromide 1 mM, 27% inhibition Microcella alkaliphila
Cu2+ 1 mM, 18% inhibition Microcella alkaliphila
Hg2+ 1 mM, 1% residual activiy Microcella alkaliphila
additional information not inhibitory at 1 mM: iodoacetate, N-ethylmaleimide, o-phenanthroline, EDTA, EGTA, dithiothreitol, 2-mercaptoethanol, 1-ethyl-3-dimethyl aminopropylcarbadimide, N-bromosuccinimide, phenylmethansulfonylfluoride, 4-chloromercuribenzoate, SDS, Tween 80, and Triton X-100. Not inhibitory: Ca2+, Sr2+, Co2+, Mg2+, Pb2+, Ni2+, Mn2+, Fe2+, Sn2+, Zn2+, and Fe3+ Microcella alkaliphila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 SDS-PAGE Microcella alkaliphila

Organism

Organism UniProt Comment Textmining
Microcella alkaliphila A0A0U5B5Z4
-
-
Microcella alkaliphila JAM-AC0309 A0A0U5B5Z4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O 148% of the activity with oat spelt xylan Microcella alkaliphila xylo-oligosaccharides + ?
-
 ?
arabinoxylan + H2O 148% of the activity with oat spelt xylan Microcella alkaliphila JAM-AC0309 xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O 110% of the activity with oat spelt xylan Microcella alkaliphila xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O 110% of the activity with oat spelt xylan Microcella alkaliphila JAM-AC0309 xylo-oligosaccharides + ?
-
 ?
additional information enzyme degrades degraded xylooligosaccharides more than xylotetraose. No substrates: avicel, carboxymethyl cellulose, lichenan, soluble starch, glucomannan, galactomannan, or arabinan, 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinopyranoside. The enzyme also catalyzes transglycosylation. Xylobiose to xylohexaose are transglycolated by xylobiose units during the early degradation step, respectively Microcella alkaliphila ?
-
 ?
additional information enzyme degrades degraded xylooligosaccharides more than xylotetraose. No substrates: avicel, carboxymethyl cellulose, lichenan, soluble starch, glucomannan, galactomannan, or arabinan, 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinopyranoside. The enzyme also catalyzes transglycosylation. Xylobiose to xylohexaose are transglycolated by xylobiose units during the early degradation step, respectively Microcella alkaliphila JAM-AC0309 ?
-
 ?
oat spelt xylan + H2O
-
 Microcella alkaliphila xylo-oligosaccharides + ?
-
 ?
oat spelt xylan + H2O
-
 Microcella alkaliphila JAM-AC0309 xylo-oligosaccharides + ?
-
 ?
xylopentaose + H2O
-
 Microcella alkaliphila xylobiose + xylotriose
-
 ?
xylopentaose + H2O
-
 Microcella alkaliphila JAM-AC0309 xylobiose + xylotriose
-
 ?

Synonyms

Synonyms Comment Organism
MalAC0309_0409
-
 Microcella alkaliphila
XynE15
-
 Microcella alkaliphila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Microcella alkaliphila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 48 h Microcella alkaliphila
75
-
 30 min, stable up to 75°C Microcella alkaliphila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Microcella alkaliphila

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 more than 80% of maximum activity Microcella alkaliphila