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Literature summary for 3.2.1.8 extracted from

  • Düsterhöft, E.-M.; Linssen, V. A. J. M.; Voragen, A. G. J. ; Beldman, G.
    Purification, characterization, and properties of two xylanases from Humicola insolens (1997), Enzyme Microb. Technol., 20, 437-445.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 41% inhibition of xylanase 2, no inhibition of xylanase 1 Humicola insolens
HgCl2 1 mM, 85% inhibition of xylanase 2, 49% inhibition of xylanase 1 Humicola insolens
MnCl2 1 mM, 29% inhibition of xylanase 2, 20% inhibition of xylanase 1 Humicola insolens
NiCl2 1 mM, 19% inhibition of xylanase 2, no inhibition of xylanase 1 Humicola insolens
Pb(NO3)2 1 mM, 43% inhibition of xylanase 2, no inhibition of xylanase 1 Humicola insolens
ZnSO4 1 mM, 19% inhibition of xylanase 2, no inhibition of xylanase 1 Humicola insolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6000
-
 xylanase 1, SDS-PAGE, mass spectrometry Humicola insolens
21000
-
 xylanase 2, SDS-PAGE Humicola insolens

Organism

Organism UniProt Comment Textmining
Humicola insolens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Humicola insolens

Purification (Commentary)

Purification (Comment) Organism
-
 Humicola insolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information xylanase 1 and xylanase 2 are both inactive toward Avicel, carboxymethylcellulose, beta-glucan, arabinan (linear and branched), and (galacto)mannans. NO activity with xylotriose Humicola insolens ?
-
 ?
xylan + H2O activity towards xylans from beech, birch, larch, and arabinoxylans from wheat is higher for xylanase 2 compared to xylanase 1 Humicola insolens ?
-
 ?
xyloheptaose + H2O
-
 Humicola insolens ?
-
 ?
xylohexaose + H2O
-
 Humicola insolens ?
-
 ?
xylopentaose + H2O
-
 Humicola insolens ?
-
 ?
xylotetraose + H2O low activity Humicola insolens 2 xylobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, xylanase 2, SDS-PAGE Humicola insolens
? x * 6000, xylanase 1, SDS-PAGE Humicola insolens

Synonyms

Synonyms Comment Organism
xylanase 1
-
 Humicola insolens
xylanase 2
-
 Humicola insolens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60 xylanase 1, xylanase 2 Humicola insolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 45 min, xylanase 2 completely loses activity, xylanase 1 retains 50% of its activity Humicola insolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 xylanase 1, xylanase 2 Humicola insolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 15 h, xylanase 2 is stable Humicola insolens
8
-
 15 h, xylanase 1 loses 46% of its activity Humicola insolens
9
-
 15 h, xylanase 1 loses 15% of its activity Humicola insolens

pI Value

Organism Comment pI Value Maximum pI Value
Humicola insolens xylanase 2, isoelectric focusing
-
 7.7
Humicola insolens xylanase 1, isoelectric focusing
-
 9