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Literature summary for 3.2.1.8 extracted from

  • Kumar, V.; Satyanarayana, T.
    Generation of xylooligosaccharides from microwave irradiated agroresidues using recombinant thermo-alkali-stable endoxylanase of the polyextremophilic bacterium Bacillus halodurans expressed in Pichia pastoris (2015), Biores. Technol., 179, 382-389.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Halalkalibacterium halodurans
dithiothreitol
-
 Halalkalibacterium halodurans

Application

Application Comment Organism
degradation agroresidues subjected to alkali and microwave irradiation for 6 min in order to expose the polysaccharide component to enzymatic hydrolysis lead to increased relase of sugars. The maximum sugar content is detected in the hydrolysate of microwave-irradiated wheat bran (6.20 mg/g substrate) followed by wheat straw (4.9 mg/g substrate) Halalkalibacterium halodurans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, yields a high titer of extracellular xylanase (502 U/ml) on induction with methanol Halalkalibacterium halodurans

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ strong inhibition Halalkalibacterium halodurans
Cu2+ strong inhibition Halalkalibacterium halodurans
Hg2+ strong inhibition Halalkalibacterium halodurans
Pb2+ strong inhibition Halalkalibacterium halodurans
Sn2+ strong inhibition Halalkalibacterium halodurans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Halalkalibacterium halodurans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Halalkalibacterium halodurans

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans M4QNR9
-
-
Halalkalibacterium halodurans TSEV1 M4QNR9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
375
-
 substrate birchwood xylan, pH 9.0, 80°C Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bagasse + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
bagasse + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
corncob + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
corncob + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
wheat bran + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
wheat bran + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?
wheat straw + H2O
-
 Halalkalibacterium halodurans xylo-oligosaccharides + ?
-
 ?
wheat straw + H2O
-
 Halalkalibacterium halodurans TSEV1 xylo-oligosaccharides + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Halalkalibacterium halodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Halalkalibacterium halodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 wild-type, half-life 35 min, recombinant glycosylated enzyme, half-life 45 min Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Halalkalibacterium halodurans