Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | stimulates at 1 mM | Saccharopolyspora pathumthaniensis | |
Urea | stimulates at 1 mM | Saccharopolyspora pathumthaniensis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Saccharopolyspora pathumthaniensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Saccharopolyspora pathumthaniensis | |
Cu2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
EDTA | slight inhibition | Saccharopolyspora pathumthaniensis | |
Fe2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
Hg2+ | complete inhibition | Saccharopolyspora pathumthaniensis | |
iodoacetic acid | - |
Saccharopolyspora pathumthaniensis | |
Na+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
Ni2+ | slight inhibition | Saccharopolyspora pathumthaniensis | |
SDS | - |
Saccharopolyspora pathumthaniensis | |
Zn2+ | slight inhibition | Saccharopolyspora pathumthaniensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saccharopolyspora pathumthaniensis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | stimulates | Saccharopolyspora pathumthaniensis | |
K+ | stimulates slightly | Saccharopolyspora pathumthaniensis | |
Rb+ | stimulates | Saccharopolyspora pathumthaniensis | |
Sn2+ | stimulates slightly | Saccharopolyspora pathumthaniensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE | Saccharopolyspora pathumthaniensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora pathumthaniensis | E1APH5 | isolated from the gut of a grass-feeding termite | - |
Saccharopolyspora pathumthaniensis S582 | E1APH5 | isolated from the gut of a grass-feeding termite | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 167fold to homogeneity | Saccharopolyspora pathumthaniensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hydrolysis of best substrate beechwood xylan by the enzyme results in formation of xylobiose and xylose as major products. The enzyme is specific for xylan acting as an endo-type xylanase. Soluble oat spelt xylan is also a good substrate, while insoluble oat spelt xylan is not. No activity with Avicel, 4-nitrophenyl beta-D-xylopyranoside, and locust bean gum | Saccharopolyspora pathumthaniensis | ? | - |
? | |
additional information | hydrolysis of best substrate beechwood xylan by the enzyme results in formation of xylobiose and xylose as major products. The enzyme is specific for xylan acting as an endo-type xylanase. Soluble oat spelt xylan is also a good substrate, while insoluble oat spelt xylan is not. No activity with Avicel, 4-nitrophenyl beta-D-xylopyranoside, and locust bean gum | Saccharopolyspora pathumthaniensis S582 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Saccharopolyspora pathumthaniensis |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the G10 glucohydrolase family | Saccharopolyspora pathumthaniensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Saccharopolyspora pathumthaniensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | activity range, profile, overview | Saccharopolyspora pathumthaniensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, 2 h, stable | Saccharopolyspora pathumthaniensis |
60 | - |
purified enzyme, half-life is 180 min | Saccharopolyspora pathumthaniensis |
70 | - |
purified enzyme, half-life is 120 min | Saccharopolyspora pathumthaniensis |
80 | - |
purified enzyme, inactivation | Saccharopolyspora pathumthaniensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharopolyspora pathumthaniensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile, overview | Saccharopolyspora pathumthaniensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | purified enzyme, stable | Saccharopolyspora pathumthaniensis |